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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Gene expression profiles of bronchoalveolar cells in pulmonary TB
Tuberculosis, Volume 88, No. 1, Year 2008
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Description
The host response to Mycobacterium tuberculosis includes macrophage activation, inflammation with increased immune effector cells, tissue necrosis, and cavity formation, and fibrosis, distortion, and bronchiectasis. To evaluate the molecular basis of the immune response in the lungs of patients with active pulmonary tuberculosis (TB), we used bronchoalveolar lavage to obtain cells at the site of infection. Affymetrix GeneChip microarrays and cDNA nylon filter microarrays interrogated gene expression in bronchoalveolar lavage (BAL) cells from 11 healthy controls and 17 patients with active pulmonary TB. We found altered gene expression for 69 genes in TB versus normal controls that included cell surface markers, cytokines, chemokines, receptors, transcription factors, and complement components. In addition, TB BAL cell gene expression patterns segregated into 2 groups: one suggestive of a T helper type 1 (Th1) cellular immune response with increased signal transducer and activator of transcription-4 (STAT-4), interferon-gamma (IFN-γ receptor), and monokine induced by IFN-γ (MIG) expression with increased IFN-γ protein levels in BAL fluid; the other group displayed characteristics of Th2 immunity with increased STAT-6, CD81, and IL-10 receptor expression. We were able to demonstrate that a Th2 presentation could change to a Th1 pattern after anti-tuberculous treatment in 1 TB patient studied serially. These gene expression data support the conclusion that pulmonary TB produces a global change in the BAL cell transcriptome with manifestations of either Th1 or Th2 immunity. © 2007 Elsevier Ltd. All rights reserved.
Authors & Co-Authors
Raju, Bindu
United States, New York
Nyc Health + Hospitals/bellevue
Hoshino, Yoshihiko
United States, New York
Nyc Health + Hospitals/bellevue
Belitskaya-Lévy, Ilana
United States, New York
Nyc Health + Hospitals/bellevue
Dawson, Rodney
South Africa, Cape Town
University of Cape Town
Ress, Stanley R.
South Africa, Cape Town
University of Cape Town
Gold, Jeffrey A.
United States, New York
Nyc Health + Hospitals/bellevue
Condos, Rany
United States, New York
Nyc Health + Hospitals/bellevue
Pine, Richard
United States, Newark
Public Health Research Institute
Brown, Stuart M.
United States, New York
Nyc Health + Hospitals/bellevue
Nolan, Anna
United States, New York
Nyc Health + Hospitals/bellevue
Rom, William N.
United States, New York
Nyc Health + Hospitals/bellevue
Weiden, Michael D.
United States, New York
Nyc Health + Hospitals/bellevue
Statistics
Citations: 67
Authors: 12
Affiliations: 3
Identifiers
Doi:
10.1016/j.tube.2007.07.003
ISSN:
14729792
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases