Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Characterisation of hepatitis C virus genotype among blood donors at the regional blood transfusion centre of Ouagadougou, Burkina Faso
Blood Transfusion, Volume 12, No. SUPPL.1, Year 2014
Notification
URL copied to clipboard!
Description
Background. Hepatitis C virus (HCV) is responsible for about 900 deaths every year in Burkina Faso. In this country, serological screening for hepatitis B and C viruses is only carried out systematically among blood donors. The aim of this study was to determine the prevalence and genotypes of HCV among blood donors using reverse transcription polymerase chain reaction (PCR) and real-time PCR, respectively. Materials and methods. Serum samples were screened for antibodies to HCV using an enzymelinked immunosorbent assay (ARCHITECT-i1000SR- ABBOTT). All the reactive samples for HCV antibodies were re-tested using a second enzyme-linked immunosorbent assay (Bio-Rad, Marnes la Coquette, France) for confirmation. RNA was detected in all the reactive samples for antibodies to HCV. HCV RNA positive samples were genotyped using the HCV Real-TM Genotype kit (Sacace Biotechnologies, Italy). Results. Among 2,200 blood donors, the prevalences of antibodies to HCV and viral RNA were 4.4% (95% confidence interval=3.5-5.3) and 1.5% (95% confidence interval=1.0-2.0), respectively. Among HCV RNA carriers, genotyping showed that HCV genotypes 2 and 3 were the most prevalent as they were detected in 18 (56.3%) and 5 (15.6%) individuals, respectively. HCV genotypes 1a and 4 were the least frequent among the blood donors. HCV mixed genotypes 2/3 and 2/4 were also detected among the blood donors. Conclusion. The prevalence of HCV found in this study is lower than previously reported prevalences. Large-scale studies are needed to obtain a better picture of the molecular epidemiology of HCV in Burkina Faso. © SIMTI Servizi Srl.
Authors & Co-Authors
Zeba, Moctar Tokèda Abdoul
Burkina Faso, Ouagadougou
University of Ouagadougou
Sanou, Mahamoudou J.
Burkina Faso, Ouagadougou
National Blood Transfusion Centre Cnts
Bisseyé, Cyrille
Burkina Faso, Ouagadougou
University of Ouagadougou
Gabon, Franceville
University of Sciences and Techniques of Masuku Ustm
Kiba, Alice
Burkina Faso, Ouagadougou
National Blood Transfusion Centre Cnts
Nagalo, Bolni Marius
Burkina Faso, Ouagadougou
University of Ouagadougou
Djigma, Florencia Wendkuuni
Burkina Faso, Ouagadougou
University of Ouagadougou
Compaoré, Tegwindé Rebeca
Burkina Faso, Ouagadougou
University of Ouagadougou
Nébié, Yacouba Koumpingnin
Burkina Faso, Ouagadougou
National Blood Transfusion Centre Cnts
Kiénou, Kisito
Burkina Faso, Ouagadougou
National Blood Transfusion Centre Cnts
Sagna, Tani
Burkina Faso, Ouagadougou
University of Ouagadougou
Pietra, Virginio
Burkina Faso, Ouagadougou
Centre Medical Saint Camille
Moret, Rémy
Burkina Faso, Ouagadougou
University of Ouagadougou
Simpore, J.
Burkina Faso, Ouagadougou
University of Ouagadougou
Burkina Faso, Ouagadougou
Centre Medical Saint Camille
Statistics
Citations: 29
Authors: 13
Affiliations: 4
Identifiers
Doi:
10.2450/2013.0089-12
ISSN:
17232007
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Burkina Faso