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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
False-negative rapid diagnostic tests for malaria and deletion of the histidine-rich repeat region of the hrp2 gene
American Journal of Tropical Medicine and Hygiene, Volume 86, No. 2, Year 2012
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Description
We identified 480 persons with positive thick smears for asexual Plasmodium falciparum parasites, of whom 454 had positive rapid diagnostic tests (RDTs) for the histidine-rich protein 2 (HRP2) product of the hrp2 gene and 26 had negative tests. Polymerase chain reaction (PCR) amplification for the histidine-rich repeat region of that gene was negative in one-half (10/22) of false-negative specimens available, consistent with spontaneous deletion. False-negative RDTs were found only in persons with asymptomatic infections, and multiplicities of infection (MOIs) were lower in persons with false-negative RDTs (both P < 0.001). These results show that parasites that fail to produce HRP2 can cause patent bloodstream infections and false-negative RDT results. The importance of these observations is likely to increase as malaria control improves, because lower MOIs are associated with false-negative RDTs and false-negative RDTs are more frequent in persons with asymptomatic infections. These findings suggest that the use of HRP2-based RDTs should be reconsidered. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Koita, Ousmane A.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako
Doumbo, Ogobara K.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Ouattara, A.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Tall, Lalla K.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Konaré, Aoua
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Diakité, Mahamadou L.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Diallo, Mouctar
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Sagara, Issaka
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Masinde, Godfred L.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
United States, New Orleans
Tulane University
Doumbo, Safiatou Niare
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Dolo, Amagana I.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Tounkara, Anatole
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Traoré, Issa
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
Mali, Bamako
University of Bamako Faculty of Medicine, Pharmacy and Odonto-stomatology
Krogstad, Donald J.
United States, New Orleans
Tulane University School of Public Health and Tropical Medicine
United States, New Orleans
Tulane University
Statistics
Citations: 286
Authors: 14
Affiliations: 4
Identifiers
Doi:
10.4269/ajtmh.2012.10-0665
ISSN:
00029637
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases