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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Comparison between allicin and fluconazole in Candida albicans biofilm inhibition and in suppression of HWP1 gene expression
Phytomedicine, Volume 19, No. 1, Year 2011
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Description
Candida albicans is an opportunistic human pathogen with the ability to differentiate and grow in filamentous forms and exist as biofilms. The biofilms are a barrier to treatment as they are often resistant to the antifungal drugs. In this study, we investigated the antifungal activity of allicin, an active compound of garlic on various isolates of C. albicans. The effect of allicin on biofilm production in C. albicans as compared to fluconazole, an antifungal drug, was investigated using the tetrazolium (XTT) reduction-dependent growth and crystal violet assays as well as scanning electron microscopy (SEM). Allicin-treated cells exhibited significant reduction in biofilm growth (p < 0.05) compared to fluconazole-treated and also growth control cells. Moreover, observation by SEM of allicin and fluconazole-treated cells confirmed a dose-dependent membrane disruption and decreased production of organisms. Finally, the expression of selected genes involved in biofilm formation such as HWP1 was evaluated by semi-quantitative RT-PCR and relative real time RT-PCR. Allicin was shown to down-regulate the expression of HWP1. © 2011 Elsevier GmbH. All rights reserved.
Authors & Co-Authors
Khodavandi, Alireza
Iran, Gachsaran
Islamic Azad University, Gachsaran Branch
Harmal, Nabil Saad
Malaysia, Serdang
Universiti Putra Malaysia
Yemen, Sana'a
Sana'a University
Alizadeh, Fahimeh
Iran, Yasuj
Islamic Azad University, Yasuj Branch
Scully, Olivia Jane
Malaysia, Serdang
Universiti Putra Malaysia
Sidik, Shiran Mohd
Malaysia, Serdang
Universiti Putra Malaysia
Othman, Fauziah Bt
Malaysia, Serdang
Universiti Putra Malaysia
Sekawi, Zamberi Bin
Malaysia, Serdang
Universiti Putra Malaysia
Ng, Kee Peng
Malaysia, Kuala Lumpur
University of Malaya Medical Centre
Chong, Pei Pei
Malaysia, Serdang
Universiti Putra Malaysia
Statistics
Citations: 64
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.phymed.2011.08.060
ISSN:
09447113
e-ISSN:
1618095X
Research Areas
Genetics And Genomics
Study Approach
Quantitative