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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Recombination following superinfection by HIV-1
AIDS, Volume 18, No. 2, Year 2004
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Description
Background: There is increasing recognition of recombinant HIV-1 strains globally, but it has been unclear whether recombination results from superinfection during untreated, chronic infection. Objective: To search for evidence of recombination and superinfection in Africa, where multiple HIV-1 subtypes facilitate identification of strains. Methods: Serial blood samples from highly exposed, chronically infected women in Nairobi's Pumwani sex workers cohort were examined. Serial, complete HIV-1 RNA sequence analyses were performed for seven untreated long-term survivors. Sequences were subjected to computational analysis. Results: One woman had evidence of both superinfection and recombination. Complete HIV-1 RNA sequences were first derived from plasma obtained in 1986, when the woman had been HIV seropositive for at least 21 months; this sequence was entirely subtype A. The sequences obtained from plasma in 1995 and 1997, however, were subtype A/C recombinants with a SimPlot demonstrating that the subtype A fragment in 1995 and 1997 was derived from the original 1986 A sequence. Heteroduplex tracking assays demonstrated that the subtype C sequences were not detectable as minor species in 1986. Conclusion: Intersubtype recombination took place between the original non-recombinant subtype A strain and the superinfecting subtype C strain in an untreated, chronically infected woman. This finding helps to explain the rising prevalence of recombinant HIV-1 worldwide. Recombination resulting from superinfection with diverse strains may pose problems for eliciting broad immune responses necessary for an effective vaccine. © 2004 Lippincott Williams & Wilkins.
Authors & Co-Authors
Fang, Guowei
United States, Albany
Wadsworth Center for Laboratories and Research
Weiser, Barbara
United States, Albany
Wadsworth Center for Laboratories and Research
United States, Albany
Albany Medical College
Kuiken, Carla L.
United States, Los Alamos
Los Alamos National Laboratory
Philpott, Sean M.
United States, Albany
Wadsworth Center for Laboratories and Research
Rowland-Jones, Sarah Louise
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Plummer, Francis Allan
Canada, Winnipeg
University of Manitoba
Kimani, Joshua
Kenya, Nairobi
University of Nairobi
Shi, Binshan
United States, Albany
Wadsworth Center for Laboratories and Research
Kaul, Rupert
Canada, Winnipeg
University of Manitoba
Kenya, Nairobi
University of Nairobi
Bwayo, Job J.
Kenya, Nairobi
University of Nairobi
Anzala, Aggrey Omu
Kenya, Nairobi
University of Nairobi
Burger, Harold
United States, Albany
Wadsworth Center for Laboratories and Research
United States, Albany
Albany Medical College
Statistics
Citations: 135
Authors: 12
Affiliations: 6
Identifiers
Doi:
10.1097/00002030-200401230-00003
Research Areas
Infectious Diseases
Study Design
Cross Sectional Study
Cohort Study
Participants Gender
Female