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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Pulmonary hypertension in human newborns with congenital diaphragmatic hernia is associated with decreased vascular expression of nitric-oxide synthase
Cell Biochemistry and Biophysics, Volume 44, No. 1, Year 2006
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Description
The molecular basis of the pathogenesis of pulmonary hypertension (PH) associated with congenital diaphragmatic hernia (CDH) is poorly understood. Variation in responses to therapeutic strategies such as nitric oxide (NO) inhalation and extracorporeal membrane oxygenation (ECMO) in patients with CDH remains a major problem in pediatric critical care. We investigated the expression pattern of NO-generating enzyme nitric-oxide synthase (NOS) (both endothelial [eNOS] and inducible [iNOS] isoforms) in the lungs of CDH patients with PH and evaluated the influence of ECMO on the expression levels of these genes in an attempt to understand the underlying molecular mechanisms. Lung autopsy specimens from 23 cases of CDH not treated by ECMO and 10 ECMO-treated CDH cases were studied and compared with 11 age-matched controls. Expression of iNOS and eNOS was assessed by immunohistochemistry and video-image analysis. Expression of iNOS in the endothelium of small pulmonary arteries (external diameter ≤200 μm) was significantly lower in CDH cases that had not received ECMO treatment (p = 0.04). ECMO-treated CDH cases did not differ from controls in iNOS expression. Alveolar macrophages (CD68+ cells), of which the number also was increased, showed significantly enhanced staining for iNOS in CDH cases (p = 0.03) compared with controls. The observed decrease in pulmonary expression of iNOS in patients with CDH suggests a potential role in the pathogenesis of pulmonary hypertension in newborns with CDH. ECMO treatment was correlated with induction of this enzyme, which may result in NO-mediated vasodilatation and thereby transiently reduce the pulmonary hypertension in CDH. © Copyright 2006 by Humana Press Inc. All rights of any nature whatsoever reserved.
Authors & Co-Authors
Shehata, Sherif Mohamed K.
Netherlands, Rotterdam
Erasmus Mc Sophia Children’s Hospital
Egypt, Tanta
Tanta University Hospitals
Sharma, Hari Shanker
Netherlands, Rotterdam
Erasmus Mc Sophia Children’s Hospital
Mooi, Wolter Jan
Netherlands, Amsterdam
Amsterdam Umc - Vrije Universiteit Amsterdam
Tibboel, Dick
Netherlands, Rotterdam
Erasmus Mc Sophia Children’s Hospital
Statistics
Citations: 46
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1385/CBB:44:1:147
ISSN:
10859195
Research Areas
Noncommunicable Diseases