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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Simultaneous determination of triterpene saponins in ginseng drugs by high-performance liquid chromatography
Chemical and Pharmaceutical Bulletin, Volume 52, No. 8, Year 2004
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Description
A HPLC method for the simultaneous determination of 11 triterpene saponins with four-type aglycones (protopanaxadiol, protopanaxatriol, ocotillol and oleanolic acid types) in Ginseng drugs was developed and validated. Using a gradient of acetonitrile and 10 mM K-phosphate buffer (pH 5.80) as the mobile phase and UV detection at 196 nm, more than 18 ginsenosides with different aglycones were separated satisfactorily within 60 min. The detection limits (signal/noise≥3) were 0.1 μg for ginsenosides Rb1, Rc, Rd, Re and Rg1, chikusetsusaponin III, and notoginsenoside R2, 0.2 μg for gisenoside Ro and chikusetsusaponin IVa, 0.3 μg for chikusetsusaponin IV, and 3 μg for majonoside R2. The calibration curve of each saponin had a correlation coefficient close to 1. Intraand interday precisions were less than 2.1% (n=5) and 3.3% (n=15), respectively. The recovery rates of extraction were in the range of 96.4-102.7% for all ginsenosides. By adopting this method, the determinations of 11 ginsenosides in three Ginseng drugs derived from Panax ginseng, Panax vietnamensis var. fuscidiscus and Panax japonicus (Japan) were achieved. © 2004 Pharmaceutical Society of Japan.
Authors & Co-Authors
Zou, Kun Zon
Japan, Toyama
University of Toyama
China, Yichang
China Three Gorges University
Cai, Shaoqing
China, Beijing
Peking University
Meselhy, M. R.
Japan, Toyama
University of Toyama
Komatsu, Katsuko
Japan, Toyama
University of Toyama
Statistics
Citations: 61
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1248/cpb.52.995
ISSN:
13475223