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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
general
The metabolic, virulence and antimicrobial resistance profiles of colonising Streptococcus pneumoniae shift after PCV13 introduction in urban Malawi
Nature Communications, Volume 14, No. 1, Article 7477, Year 2023
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Description
Streptococcus pneumoniae causes substantial mortality among children under 5-years-old worldwide. Polysaccharide conjugate vaccines (PCVs) are highly effective at reducing vaccine serotype disease, but emergence of non-vaccine serotypes and persistent nasopharyngeal carriage threaten this success. We investigated the hypothesis that following vaccine, adapted pneumococcal genotypes emerge with the potential for vaccine escape. We genome sequenced 2804 penumococcal isolates, collected 4-8 years after introduction of PCV13 in Blantyre, Malawi. We developed a pipeline to cluster the pneumococcal population based on metabolic core genes into “Metabolic genotypes” (MTs). We show that S. pneumoniae population genetics are characterised by emergence of MTs with distinct virulence and antimicrobial resistance (AMR) profiles. Preliminary in vitro and murine experiments revealed that representative isolates from emerging MTs differed in growth, haemolytic, epithelial infection, and murine colonisation characteristics. Our results suggest that in the context of PCV13 introduction, pneumococcal population dynamics had shifted, a phenomenon that could further undermine vaccine control and promote spread of AMR. © 2023, The Author(s).
Authors & Co-Authors
Obolski, Uri
Israel, Tel Aviv-yafo
Tel Aviv University
Swarthout, Todd D.
Malawi, Blantyre
Malawi-liverpool-wellcome Trust Clinical Research Programme
United Kingdom, London
University College London
Netherlands, Utrecht
University Medical Center Utrecht
Kalizang’oma, Akuzike
Malawi, Blantyre
Malawi-liverpool-wellcome Trust Clinical Research Programme
United Kingdom, London
University College London
Mwalukomo, Thandie S.
Malawi, Blantyre
Kamuzu University of Health Sciences
Chan, Jia Mun
United Kingdom, London
University College London
Weight, Caroline M.
United Kingdom, London
University College London
United Kingdom, Lancaster
Lancaster University
Brown, Comfort
Malawi, Blantyre
Malawi-liverpool-wellcome Trust Clinical Research Programme
Cave, Rory J.
United Kingdom, London
University College London
Cornick, Jennifer E.
Malawi, Blantyre
Malawi-liverpool-wellcome Trust Clinical Research Programme
United Kingdom, Liverpool
University of Liverpool
Kamng’ona, Arox Wadson
Malawi, Blantyre
Kamuzu University of Health Sciences
Msefula, Jacquline
Malawi, Blantyre
Kamuzu University of Health Sciences
Ercoli, Giuseppe
United Kingdom, London
University College London
Brown, Jeremy Stuart
United Kingdom, London
University College London
Lourenço, José M.L.
United Kingdom, Oxford
University of Oxford
Portugal, Lisbon
Universidade Católica Portuguesa
Maiden, Martin Christopher James
United Kingdom, Oxford
University of Oxford
French, N.
United Kingdom, Liverpool
University of Liverpool
Gupta, Sunetra
United Kingdom, Oxford
University of Oxford
Heyderman, Robert Simon
Malawi, Blantyre
Malawi-liverpool-wellcome Trust Clinical Research Programme
United Kingdom, London
University College London
Statistics
Citations: 3
Authors: 18
Affiliations: 9
Identifiers
Doi:
10.1038/s41467-023-43160-y
ISSN:
20411723
Research Areas
Genetics And Genomics
Maternal And Child Health
Noncommunicable Diseases
Study Design
Cross Sectional Study
Study Locations
Malawi