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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
SDS Interferes with SaeS Signaling of Staphylococcus aureus Independently of SaePQ
PLoS ONE, Volume 8, No. 8, Article e71644, Year 2013
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Description
The Staphylococcus aureus regulatory saePQRS system controls the expression of numerous virulence factors, including extracellular adherence protein (Eap), which amongst others facilitates invasion of host cells. The saePQRS operon codes for 4 proteins: the histidine kinase SaeS, the response regulator SaeR, the lipoprotein SaeP and the transmembrane protein SaeQ. S. aureus strain Newman has a single amino acid substitution in the transmembrane domain of SaeS (L18P) which results in constitutive kinase activity. SDS was shown to be one of the signals interfering with SaeS activity leading to inhibition of the sae target gene eap in strains with SaeSL but causing activation in strains containing SaeSP. Here, we analyzed the possible involvement of the SaeP protein and saePQ region in SDS-mediated sae/eap expression. We found that SaePQ is not needed for SDS-mediated SaeS signaling. Furthermore, we could show that SaeS activity is closely linked to the expression of Eap and the capacity to invade host cells in a number of clinical isolates. This suggests that SaeS activity might be directly modulated by structurally non-complex environmental signals, as SDS, which possibly altering its kinase/phosphatase activity. © 2013 Makgotlho et al.
Authors & Co-Authors
Makgotlho, Phuti Edward
Germany, Wurzburg
Julius-maximilians-universität Würzburg
Marincola, Gabriella
Germany, Tubingen
Eberhard Karls Universität Tübingen
Schäfer, Daniel
Germany, Wurzburg
Julius-maximilians-universität Würzburg
Liu, Qian
United States, Gary
Indiana University School of Medicine-gary
Bae, Taeok
United States, Gary
Indiana University School of Medicine-gary
Geiger, Tobias
Germany, Tubingen
Eberhard Karls Universität Tübingen
Wasserman, Elizabeth
South Africa, Stellenbosch
Stellenbosch University
Wolz, Christiane
Germany, Tubingen
Eberhard Karls Universität Tübingen
Ziebuhr, Wilma
Germany, Wurzburg
Julius-maximilians-universität Würzburg
Sinha, Bhanu N.M.
Netherlands, Groningen
Universitair Medisch Centrum Groningen
Statistics
Citations: 12
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0071644
e-ISSN:
19326203
Research Areas
Genetics And Genomics