Molecular beacon structure mediated rolling circle amplification for ultrasensitive electrochemical detection of microRNA based on quantum dots tagging

MicroRNA-16 (miR-16) frequently deletedor downregulatedin patients withB cell chronic lymphocytic leukemia and thus can be used as promising biomarkers in B-CLL diagnosis and prognosis. Herein, an ultrasensitive miR-16 detection was carried out by combining rolling circle amplification (RCA), quantum dots tagging and anodic stripping voltammetric detection (ASV). The method could specifically quantify miR-16 over a 6-decade dynamic range and reach an ultra-low detection limit of 0.32 aM. © 2013 Elsevier B.V. All rights reserved.