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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Novel thalidomide analogs: Anti-angiogenic and apoptotic effects on Hep-G2 and MCF-7 cancer cell lines
Biomedicine and Aging Pathology, Volume 4, No. 3, Year 2014
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Description
Thalidomide is one of the anti-angiogenic drugs, which have been investigated as a possible treatment for various cancers and diseases. This work aimed to study novel thalidomide analogs for their anti-cytotoxicity, anti-angiogenic, treratogenic gene (FGF-2) expression as well as changes in histone deacetylase (HDAC) activity and nuclear factor kappa-B (NF-κB) level using two different cancer cell lines (Hep-G2 and MCF-7 cells). MTT assay was used to assess the cytotoxic effect of thalidomide analogs against Hep-G2 and MCF-7 cells. HDAC activity was estimated by colorimetric assay. NFκB- P65, pro-angiogenesis and anti-angiogenesis markers were determined by Enzyme-linked immunosorbent assay (ELISA). FGF-2 expression was confirmed by reverse transcription-polymerase chain reaction (RT-PCR). Thalidomide dithiocarbamate analogs 1, 5 and thalidomide dithioate analog 3 showed elevation in their cytotoxic activity better than thalidomide. Thalidomide dithioate analog 2 and thalidomide dithiocarbamate analog 4 showed reduction in pro-angiogenic and elevation of anti-angiogenic factors in both cell lines; furthermore, analog 1 is the most potent analog in MCF-7 cells as an anti-angiogenic agent. In Hep-G2 cells, analogs 1, 2, 4 showed a significant increase while analogs 3 and 5 induced a significant decrease in NF-κB level in relation to thalidomide. A drastic decline in HDAC activity was demonstrated in the following order 2 > thalidomide > 1 > 3 > 5 > 4 of the control activity. In conclusion, this study showed that thalidomide analogs are more potent anti-tumor agents with more pronounced effect by working selectively on specific types of tumors than thalidomide. © 2014 Elsevier Masson SAS.
Authors & Co-Authors
Talaat, Roba Mohamed
Egypt, Sadat City
University of Sadat City
Elsayed, Waheba
Egypt, Sadat City
University of Sadat City
Agwa, Hussein S.
Egypt, Shibin el Kom
Faculty of Science
Gamal-Eldeen, Amira
Egypt, Giza
National Research Centre
Moawia, Shaden
Egypt, Sadat City
University of Sadat City
Zahran, Magdy Abdel Hamied
Egypt, Shibin el Kom
Faculty of Science
Statistics
Citations: 15
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.1016/j.biomag.2014.03.002
Research Areas
Cancer
Genetics And Genomics