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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
The prevalence of resistance-associated mutations to protease and reverse transcriptase inhibitors in treatment-naïve (HIV1)-infected individuals in Casablanca, Morocco
Journal of Infection in Developing Countries, Volume 3, No. 5, Year 2009
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Description
Background: The widespread use of antiretroviral agents and the growing occurrence of HIV-1 strains resistant to these drugs have given rise to serious concerns regarding the transmission of resistant viruses to newly infected persons, which may reduce the efficacy of a first-line antiretroviral therapy. Methodology: RNA was extracted from plasma samples of 98 treatment-naïve individuals with a plasma HIV RNA viral load of at least 1,000 copies/ml. Both protease (pr) and reverse transcriptase (rt) were amplified and sequenced using an automated sequencer. National Agency for AIDS Research (ANRS) and Stanford HIV database algorithms were used for interpretation of resistance data. Results: In the protease segment, various minor mutations were present in the majority of the sequenced samples with high frequencies. Only two major mutations, M46L and V82L, were separately found in three individuals of 71 (4.2%) with one carrying both mutations. In the reverse transcriptase gene, no NNRTIs-associated resistance mutations were detected. Only one patient of 70 (1.4%) carried the F77L mutation that is associated with NRTIs resistance. Genetic subtyping revealed that 74.6% of samples were infected with subtype B, 15.5% with CRF02_AG, 4.2% with CRF01Æ, 1.4% with C, 2.8% with G and 1.4% with subtype F2. Conclusions: The low prevalence of major mutations associated with resistance to antiretroviral drugs (ARVs) among drug-naïve individuals studied suggests that the routine of drug resistance testing may be unnecessary for all Moroccan individuals newly diagnosed or all patients beginning antiretroviral therapy. Nevertheless, continuous surveillance is required since greater access to antiretroviral drugs is expected in Morocco. Copyright © 2009 Bakhouch et al.
Authors & Co-Authors
Bakhouch, Khadija
Morocco, Casablanca
Institut Pasteur du Maroc
Oulad-Lahcen, Ahd
Morocco, Casablanca
Centre Hospitalier Universitaire Ibn Rochd
Bensghir, Rajaa
Morocco, Casablanca
Centre Hospitalier Universitaire Ibn Rochd
Blaghen, Mohamed Nabil Benchekrouir
Morocco, Casablanca
Faculté Des Sciences Aïn Chock
Elfilali, Kamal Marhoum
Morocco, Casablanca
Institut Pasteur du Maroc
Ezzikouri, Sayeh
Morocco, Casablanca
Institut Pasteur du Maroc
Abidi, Omar
Morocco, Casablanca
Institut Pasteur du Maroc
Hassar, Mohammed
Morocco, Casablanca
Institut Pasteur du Maroc
Wakrim, Lahcen
Morocco, Casablanca
Institut Pasteur du Maroc
Statistics
Citations: 33
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.3855/jidc.247
Research Areas
Cancer
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Design
Cross Sectional Study
Study Locations
Morocco