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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Simultaneous quantification of carbamate insecticides in human plasma by liquid chromatography/tandem mass spectrometry
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Volume 879, No. 23, Year 2011
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Description
Carbofuran (CFN), carbosulfan (CSN) and fenobucarb (FBC) are carbamate pesticides that are widely used in gardening and agriculture for the control of insects. Human poisoning due to occupational or self-poisoning exposures is also reported, so assays are required to quantify the plasma concentration of these insecticides. An LC-MS/MS method was developed and validated for the simultaneous quantification of these three carbamate insecticides in the plasma of patients with acute intentional self-poisoning. Plasma samples were pretreated by acetonitrile for protein precipitation. Chromatography was carried out on a Luna C18(2) analytical column with gradient elution using a mobile phase containing acetonitrile and water with 10. mM ammonium acetate. Mass spectrometric analysis was performed by an Applied Biosystems MDS Sciex API 2000 triple quadrupole mass spectrometer coupled with electrospray ionization (ESI) source in the positive ion mode. The total run time was 7. min. The assay was validated over a concentration range from 10 to 1000. ng/ml for CSN and FBC and 20-2000. ng/ml for CFN. The precision and accuracy for both intra- and inter-day determination of all analytes were acceptable (<15%). No significant matrix effect was observed. Stability of compounds was established for short term bench and autosampler storage as well as freeze/thaw cycles. The method was effectively applied to 270 clinical samples from patients with a history of acute intentional carbamate self-poisoning. © 2011 Elsevier B.V.
Authors & Co-Authors
Mostafa, Ahmed A.
Australia, Brisbane
The University of Queensland
Egypt, Helwan
Faculty of Pharmacy
Medley, Gregory A.
Australia, Brisbane
The University of Queensland
Roberts, Darren M.
Sri Lanka, Peradeniya
University of Peradeniya
Australia, Brisbane
The University of Queensland
Mohamed, M. S.
Egypt, Helwan
Faculty of Pharmacy
Elshanawane, Abdalla Ahmed
Egypt, Zagazig
Faculty of Pharmacy
Roberts, Michael S.
Australia, Brisbane
The University of Queensland
Australia, Adelaide
University of South Australia
Liu, Xin
Australia, Brisbane
The University of Queensland
Statistics
Citations: 14
Authors: 7
Affiliations: 5
Identifiers
Doi:
10.1016/j.jchromb.2011.06.006
ISSN:
15700232
e-ISSN:
1873376X
Research Areas
Environmental