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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Apoptosis role of FAS/FAS ligand system in the regulation of myelopoiesis
Yale Journal of Biology and Medicine, Volume 78, No. 1, Year 2005
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Description
Objectives: CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis. Methods: Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony assay and the proliferative capacity of CFU-GM was measured by replating the primary colony and observing the secondary colony formation. Human marrow cells were treated with IETD (caspase-8 inhibitor) or anti-Fas CH-11 Mab. Results: Treatment with the CFU-GM with IETD significantly increased, the proliferative capacity, while anti-Fas CH-11 Mab markedly reduced it. Fas and FasL expression were demonstrated using RT-PCR and immunofluorescence respectively. Conclusion: Fas, FasL, and caspase activation are likely to play an important role in the regulation of myelopoiesis. Copyright © 2005. All rights reserved.
Authors & Co-Authors
Alenzi, Faris Q.B.
Saudi Arabia, Dammam
Imam Abdulrahman Bin Faisal University
Al-Ghamdi, Saleh M.
Saudi Arabia, Riyadh
King Abdulaziz Medical City - Riyadh
Tamimi, Waleed G.
Saudi Arabia, Riyadh
King Abdulaziz Medical City - Riyadh
Sebiany, Abdulaziz
Saudi Arabia, Dammam
Imam Abdulrahman Bin Faisal University
El-Nashar, Iman M.
Saudi Arabia, Dammam
Imam Abdulrahman Bin Faisal University
El-Tounsi, Iman
Egypt, Shibin el Kom
Menoufia University Faculty of Medicine
Bamaga, Mohammad S.
Saudi Arabia, Taif
Military Forces Hospital
Al-Enazi, Maher M.
Saudi Arabia, Riyadh
College of Sciences
Al-Amri, Ali S.
Saudi Arabia, Dammam
Imam Abdulrahman Bin Faisal University
Al-Sheikh, Iman
Saudi Arabia, Dammam
Imam Abdulrahman Bin Faisal University
Statistics
Citations: 10
Authors: 10
Affiliations: 5
Identifiers
ISSN:
00440086
Research Areas
Cancer