Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes
Nature Communications, Volume 5, Article 4498, Year 2014
Notification
URL copied to clipboard!
Description
Metagenomics, or sequencing of the genetic material from a complete microbial community, is a promising tool to discover novel microbes and viruses. Viral metagenomes typically contain many unknown sequences. Here we describe the discovery of a previously unidentified bacteriophage present in the majority of published human faecal metagenomes, which we refer to as crAssphage. Its ∼97kbp genome is six times more abundant in publicly available metagenomes than all other known phages together; it comprises up to 90% and 22% of all reads in virus-like particle (VLP)-derived metagenomes and total community metagenomes, respectively; and it totals 1.68% of all human faecal metagenomic sequencing reads in the public databases. The majority of crAssphage-encoded proteins match no known sequences in the database, which is why it was not detected before. Using a new co-occurrence profiling approach, we predict a Bacteroides host for this phage, consistent with Bacteroides-related protein homologues and a unique carbohydrate-binding domain encoded in the phage genome. © 2014 Macmillan Publishers Limited.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4111155/bin/ncomms5498-s1.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4111155/bin/ncomms5498-s2.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC4111155/bin/ncomms5498-s3.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC4111155/bin/ncomms5498-s4.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC4111155/bin/ncomms5498-s5.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC4111155/bin/ncomms5498-s6.xlsx
Authors & Co-Authors
Dutilh, Bas E.
Netherlands, Nijmegen
Radboud Institute for Molecular Life Sciences
United States, San Diego
San Diego State University
Brazil, Rio de Janeiro
Universidade Federal do Rio de Janeiro
Cassman, Noriko
United States, San Diego
San Diego State University
Netherlands, Wageningen
Netherlands Institute of Ecology Nioo - Knaw
McNair, Katelyn
United States, San Diego
San Diego State University
Sanchez, Savannah E.
United States, San Diego
San Diego State University
Silva, Genivaldo Gueiros Zacarias
United States, San Diego
San Diego State University
Boling, Lance
United States, San Diego
San Diego State University
Barr, Jeremy J.
United States, San Diego
San Diego State University
Speth, Daan R.
Netherlands, Nijmegen
Radboud Universiteit
Seguritan, Victor
United States, San Diego
San Diego State University
Aziz, Ramy Karam
United States, San Diego
San Diego State University
Egypt, Cairo
Faculty of Pharmacy
Felts, Ben
United States, San Diego
San Diego State University
Dinsdale, Elizabeth Ann
United States, San Diego
San Diego State University
Mokili, John L.K.
United States, San Diego
San Diego State University
Edwards, Robert A.
United States, San Diego
San Diego State University
Brazil, Rio de Janeiro
Universidade Federal do Rio de Janeiro
United States, Lemont
Argonne National Laboratory
Statistics
Citations: 586
Authors: 14
Affiliations: 7
Identifiers
Doi:
10.1038/ncomms5498
e-ISSN:
20411723
Research Areas
Genetics And Genomics