Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Feedback inhibition of starch degradation in Arabidopsis leaves mediated by trehalose 6-phosphate
Plant Physiology, Volume 163, No. 3, Year 2013
Notification
URL copied to clipboard!
Description
Many plants accumulate substantial starch reserves in their leaves during the day and remobilize them at night to provide carbon and energy for maintenance and growth. In this paper, we explore the role of a sugar-signaling metabolite, trehalose-6-phosphate (Tre6P), in regulating the accumulation and turnover of transitory starch in Arabidopsis (Arabidopsis thaliana) leaves. Ethanol-induced overexpression of trehalose-phosphate synthase during the day increased Tre6P levels up to 11-fold. There was a transient increase in the rate of starch accumulation in the middle of the day, but this was not linked to reductive activation of ADP-glucose pyrophosphorylase. A 2- to 3-fold increase in Tre6P during the night led to significant inhibition of starch degradation. Maltose and maltotriose did not accumulate, suggesting that Tre6P affects an early step in the pathway of starch degradation in the chloroplasts. Starch granules isolated from induced plants had a higher orthophosphate content than granules from noninduced control plants, consistent either with disruption of the phosphorylation-dephosphorylation cycle that is essential for efficient starch breakdown or with inhibition of starch hydrolysis by β-amylase. Nonaqueous fractionation of leaves showed that Tre6P is predominantly located in the cytosol, with estimated in vivo Tre6P concentrations of 4 to 7 μM in the cytosol, 0.2 to 0.5 μM in the chloroplasts, and 0.05 μM in the vacuole. It is proposed that Tre6P is a component in a signaling pathway that mediates the feedback regulation of starch breakdown by sucrose, potentially linking starch turnover to demand for sucrose by growing sink organs at night. © 2013 American Society of Plant Biologists. All Rights Reserved.
Authors & Co-Authors
Martins, Marina Camara Mattos
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Hejazi, Mahdi Mehdi
Germany, Potsdam
Universität Potsdam
Brazil, Campinas
Laboratório Nacional de Biorrenováveis
Fettke, Joerg
Germany, Potsdam
Universität Potsdam
Steup, Martin
Germany, Potsdam
Universität Potsdam
Feil, Regina
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Krause, Ursula
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Arrivault, Stéphanie
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Vosloh, Daniel
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
South Africa, Stellenbosch
Stellenbosch University
Figueroa, Carlos María
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Ivakov, Alexander A.
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Yadav, Umesh Prasad
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
United States, Denton
University of North Texas
Piques, Maria Conceição
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Metzner, Daniela
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Stitt, Mark
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Lunn, John Edward
Germany, Potsdam
Max Planck Institute of Molecular Plant Physiology
Statistics
Citations: 165
Authors: 15
Affiliations: 5
Identifiers
Doi:
10.1104/pp.113.226787
ISSN:
00320889
e-ISSN:
15322548
Research Areas
Environmental