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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Rapid activation of FAK/mTOR/p70S6K/PAK1-signaling controls the early testosterone-induced actin reorganization in colon cancer cells
Cellular Signalling, Volume 25, No. 1, Year 2013
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Description
Actin cytoskeleton reorganization initiated by testosterone conjugates through activation of membrane androgen receptors (mAR) has recently been reported in colon tumor cells. This mAR-induced actin reorganization was recognized as a critical initial event, controlling apoptosis and inhibiting cell migration. The present study addressed the molecular signaling regulating the rapid actin remodeling initiated upon testosterone-induced mAR activation in Caco2 colon tumor cells. We report early phosphorylation of the Focal Adhesion Kinase (FAK), followed by substantial early phosphorylation of mammalian target of rapamycin (mTOR), S6 kinase (p70S6K) and the actin regulating p21-activated kinase (PAK1). Pharmacological inhibition of FAK-sensitive phosphatidylinositide-3-kinase (PI-3K), a known element of mAR-signaling, fully abrogated the testosterone-induced actin reorganization and the activation of mTOR, p70S6K and PAK1. Similarly, inhibition of mTOR blocked p70S6K and PAK1 phosphorylation and actin remodeling. Pretreatment of the cells with the intracellular androgen receptor (iAR) antagonist flutamide or silencing iAR through siRNA did not influence mTOR phosphorylation and actin reorganization, indicating specific mAR-induced testosterone effects that are independent of iAR signaling. In conclusion, we demonstrate for the first time a new mAR-governed pathway involving FAK/PI-3K and mTOR/p70S6K/PAK1-cascade that regulates early actin reorganization in colon cancer cells. © 2012 Elsevier Inc.
Authors & Co-Authors
Gu, Shuchen
Germany, Tubingen
Eberhard Karls Universität Tübingen
Kounenidakis, Michalis
Greece, Heraklion
University of Crete Medical School
Schmidt, Eva Maria
Germany, Tubingen
Eberhard Karls Universität Tübingen
Deshpande, Divija
Germany, Tubingen
Eberhard Karls Universität Tübingen
Alkahtani, Saad Hussin
Saudi Arabia, Riyadh
King Saud University
Alarifi, Saud A.
Saudi Arabia, Riyadh
College of Sciences
Föller, Michael
Germany, Tubingen
Eberhard Karls Universität Tübingen
Canada, Toronto
Princess Margaret Cancer Centre
Alevizopoulos, Konstantinos
Greece, Heraklion
University of Crete Medical School
Lang, Florian C.
Germany, Tubingen
Eberhard Karls Universität Tübingen
Stournaras, Christos
Germany, Tubingen
Eberhard Karls Universität Tübingen
Greece, Heraklion
University of Crete Medical School
Statistics
Citations: 46
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1016/j.cellsig.2012.08.005
ISSN:
08986568
e-ISSN:
18733913
Research Areas
Cancer