Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Single nucleotide polymorphism typing of mycobacterium ulcerans reveals focal transmission of buruli ulcer in a highly endemic Region of Ghana
PLoS Neglected Tropical Diseases, Volume 4, No. 7, Article e751, Year 2010
Notification
URL copied to clipboard!
Description
Buruli ulcer (BU) is an emerging necrotizing disease of the skin and subcutaneous tissue caused by Mycobacterium ulcerans. While proximity to stagnant or slow flowing water bodies is a risk factor for acquiring BU, the epidemiology and mode of M. ulcerans transmission is poorly understood. Here we have used high-throughput DNA sequencing and comparisons of the genomes of seven M. ulcerans isolates that appeared monomorphic by existing typing methods. We identified a limited number of single nucleotide polymorphisms (SNPs) and developed a real-time PCR SNP typing method based on these differences. We then investigated clinical isolates of M. ulcerans on which we had detailed information concerning patient location and time of diagnosis. Within the Densu river basin of Ghana we observed dominance of one clonal complex and local clustering of some of the variants belonging to this complex. These results reveal focal transmission and demonstrate, that micro-epidemiological analyses by SNP typing has great potential to help us understand how M. ulcerans is transmitted. © 2010 Röltgen et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2907412/bin/pntd.0000751.s001.tif
https://efashare.b-cdn.net/share/pmc/articles/PMC2907412/bin/pntd.0000751.s002.doc
Authors & Co-Authors
Röltgen, Katharina
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
Universitat Basel
Qi, Weihong
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
Universitat Basel
Switzerland, Zurich
Functional Genomics Center Zurich
Ruf, Marie Thérèse
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
Universitat Basel
Mensah-Quainoo, Ernestina
Ghana, Accra
Ministry of Health Ghana
Pidot, Sacha J.
Australia, Clayton
Monash University
Seemann, Torsten
Australia, Clayton
Monash University
Stinear, Timothy Paul
Australia, Clayton
Monash University
Käser, Michael
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
Universitat Basel
Ghana, Accra
School of Public Health
Yeboah-Manu, Dorothy
Ghana, Accra
Noguchi Memorial Institute for Medical Research
Pluschke, Gerd
Switzerland, Allschwil
Swiss Tropical and Public Health Institute Swiss Tph
Switzerland, Basel
Universitat Basel
Statistics
Citations: 79
Authors: 10
Affiliations: 7
Identifiers
Doi:
10.1371/journal.pntd.0000751
Research Areas
Environmental
Genetics And Genomics
Health System And Policy
Study Locations
Ghana