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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Cross-reactive cytotoxic T lymphocytes against a HIV-1 p24 epitope in slow progressors with B*57
AIDS, Volume 16, No. 7, Year 2002
Notification
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Description
Objectives: To determine whether CD8 T lymphocytes from HIV-1-infected patients expressing B*5701 and B*5703 show broad cross-reactivity against different variants of a conserved p24 epitope, which might account for the good prognosis of HIV-1-infected individuals with HLA-B*57. Design: B*5701+ and B*5703+ were recruited from Nairobi, Kenya and from Oxford, UK. All patients had been HIV positive for at least 8 years and could be categorized as slow progressors. Methods: CD8 cytotoxic T cell clones were generated from B*5701+ and B*5703+ donors and tested for their ability to recognize clade variants of an index p24 epitope in standard cytolytic assays. Cross-reactive responses in freshly isolated peripheral blood mononuclear cells (PBMC) were assessed by interferon-γ (IFNγ) production and tetramer binding. Results: Broad cross-clade reactivity for both cytolysis and tetramer binding was observed in CD8 T cell clones from patients harbouring the index epitope sequence. Patterns of cross-reactivity were similar in freshly isolated PBMC but varied between individuals in terms of strength and breath of responses generated. One common variant induced an unusual response with tetramer binding but often failed to induce IFNγ production, and another was a weak stimulator of both IFNγ and cytolytic activity. Conclusion: B*5701+ and B5703+ donors demonstrate broad functional cross-reactivity to both common and rare variants of a dominant p24 epitope, which could be relevant to the association of B*57 alleles with slow progression to AIDS. © 2002 Lippincott Williams & Wilkins.
Authors & Co-Authors
Gillespie, Geraldine M.A.
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Kaul, Rupert
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Dong, Tao
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Yang, Hongbing
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Rostron, Tim
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Bwayo, Job J.
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Kiama, Peter
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Peto, Timothy E.A.
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Plummer, Francis Allan
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
McMichael, Andrew James
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Rowland-Jones, Sarah Louise
United Kingdom, Oxford
Mrc Weatherall Institute of Molecular Medicine
Statistics
Citations: 102
Authors: 11
Affiliations: 1
Identifiers
Doi:
10.1097/00002030-200205030-00002
ISSN:
02699370
Research Areas
Infectious Diseases
Study Locations
Kenya