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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Understanding bovine trypanosomiasis and trypanotolerance: The promise of functional genomics
Veterinary Immunology and Immunopathology, Volume 105, No. 3-4, Year 2005
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Description
African bovine trypanosomiasis, caused by the protozoan parasite Trypanosoma congolense, is endemic throughout sub-Saharan Africa and is a major constraint on livestock production. A promising approach to disease control is to understand and exploit naturally evolved trypanotolerance. We describe the first attempt to investigate the transcriptional response of susceptible Boran (Bos indicus) cattle to trypanosome infection via a functional genomics approach using a bovine total leukocyte (BOTL) cDNA microarray platform. Four male Boran cattle were experimentally infected with T. congolense and peripheral blood mononuclear cells (PBMC) were collected before infection and 13, 17, 23 and 30 days post-infection (dpi). A reference experimental design was employed using a universal bovine reference RNA pool. Data were normalised to the median of a set of invariant genes (GAPDH) and BRB-Array tools was used to search for statistically significant differentially expressed genes between each time-point. Using a set of 20 microarray hybridisations, we have made a significant contribution to understand the temporal transcriptional response of bovine PBMC in vivo to a controlled trypanosome infection. The greatest changes were evident 13 dpi after parasites were first detected in the blood. Significant differences were observed in clusters of protein kinase C subunits and MHC class I/II related molecules. © 2005 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Hill, Emmeline W.
Ireland, Dublin
University College Dublin
O'Gorman, Grace M.
Ireland, Dublin
University College Dublin
Agaba, Morris K.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Gibson, J. P.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Australia, Armidale
University of new England Australia
Hanotte, Olivier H.
Kenya, Nairobi
International Livestock Research Institute Nairobi
Kemp, Stephen J.
United Kingdom, Liverpool
University of Liverpool
Naessens, Jan
Kenya, Nairobi
International Livestock Research Institute Nairobi
Coussens, Paul M.
United States, East Lansing
Michigan State University
MacHugh, David E.
Ireland, Dublin
University College Dublin
Statistics
Citations: 70
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.1016/j.vetimm.2005.02.004
ISSN:
01652427
Research Areas
Cancer
Genetics And Genomics
Participants Gender
Male