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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR
Transactions of the Royal Society of Tropical Medicine and Hygiene, Volume 103, No. 4, Year 2009
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Description
A real-time PCR method targeting the small subunit of the rRNA gene was developed for the detection of Strongyloides stercoralis DNA in faecal samples, including an internal control to detect inhibition of the amplification process. The assay was performed on a range of well-defined control samples (n = 145), known positive faecal samples (n = 38) and faecal samples from a region in northern Ghana where S. stercoralis infections are highly endemic (n = 212), and achieved 100% specificity and high sensitivity. The use of this assay could facilitate monitoring the prevalence and intensity of S. stercoralis infections during helminth intervention programs. Moreover, the use of this assay in diagnostic laboratories could make the introduction of molecular diagnostics feasible in the routine diagnosis of S. stercoralis infections, with a two-fold increase in the detection rate as compared with the commonly used Baermann sedimentation method. © 2008 Royal Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Verweij, Jaco J.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Canales, Marco
Peru, Lima
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt
Polman, Katja
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Ziem, Juventus Benogle
Ghana, Tamale
University for Development Studies Ghana
Brienen, Eric A.T.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Polderman, Anton M.
Netherlands, Leiden
Leids Universitair Medisch Centrum
van Lieshout, Lisette
Netherlands, Leiden
Leids Universitair Medisch Centrum
Statistics
Citations: 294
Authors: 7
Affiliations: 4
Identifiers
Doi:
10.1016/j.trstmh.2008.12.001
ISSN:
00359203
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Randomised Control Trial
Cross Sectional Study
Study Locations
Ghana