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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Evaluation of the Genotype® MTBDRplus assay as a tool for drug resistance surveys
International Journal of Tuberculosis and Lung Disease, Volume 15, No. 7, Year 2011
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Description
SETTING: A national tuberculosis (TB) drug resistance survey in Tanzania. OBJECTIVE: To compare the performance of the Genotype® MTBDRplus line-probe assay (LPA) on smear-positive sputum specimens with conventional culture and isoniazid (INH) plus rifampicin (RMP) drug susceptibility testing (DST). DESIGN: Mycobacterium tuberculosis isolates tested at the Tanzanian Central TB Reference Laboratory (CTRL) were submitted for quality assurance of phenotypic DST to its supranational reference laboratory (SRL), together with ethanol-preserved sputum specimens for LPA DST. RESULTS: Only 321 samples could be tested using LPA; of these, three were identified as being non-tuberculous mycobacteria using CTRL DST. Both tests had 269 sets with interpretable results. CTRL DST yielded almost the same number of interpretable results as LPA, with 90% concordance (κ = 0.612, P < 0.001). Five (1.9%) multidrug-resistant (MDR) strains, 46 (17.1%) resistant to INH only and 0 RMP only, were found by CTRL DST. For the LPA, these results were respectively 5 (1.9%), 26 (9.7%) and 2 (0.7%). With SRL DST as the gold standard, LPA was more accurate than CTRL DST for RMP, but missed almost half the INH-resistant samples. CONCLUSION: LPA applied directly on ethanol-preserved sputum specimens was similar to phenotypic DST in terms of yield of interpretable results. Although probably more accurate for RMP and MDR-TB, it appears to seriously underestimate INH resistance. Considering speed, easy and safe specimen transportation and low infrastructure requirements, LPA DST from sputum can be recommended for surveys in resource-poor settings. © 2011 The Union.
Authors & Co-Authors
Rigouts, Leen
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Belgium, Antwerpen
Universiteit Antwerpen
Hoza, Abubakar Shaaban
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
de Rijk, Pim B.
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Torrea, Gabriela
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Chonde, Timothy
Tanzania, Dar es Salaam
Central Tuberculosis Reference Laboratory, Dar-es-salaam
Basra, D.
Tanzania, Dar es Salaam
Central Tuberculosis Reference Laboratory, Dar-es-salaam
Zignol, Matteo
Switzerland, Geneva
Organisation Mondiale de la Santé
van Leth, Frank C.M.
Netherlands, The Hague
Kncv Tuberculosis Foundation
Netherlands, Amsterdam
Universiteit Van Amsterdam
Egwaga, Saidi M.
Tanzania, Dar es Salaam
Ministry of Health and Social Welfare
van Deun, Armand
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
France, Paris
International Union Against Tuberculosis and Lung Disease
Statistics
Citations: 27
Authors: 10
Affiliations: 8
Identifiers
Doi:
10.5588/ijtld.10.0515
ISSN:
10273719
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Tanzania