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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites
Molecular and Biochemical Parasitology, Volume 51, No. 1, Year 1992
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Description
We have cloned and characterised the gene encoding the 67-kilodalton stage-specific surface antigen, p67, of Theileria parva (Muguga) sporozoites. The gene which is present in a single copy, is divided into 2 exons by an intron 29 bp long and is transcribed into mRNA of about 2500 nucleotides. The gene is present in all stocks of T.parva and there is a related gene in Theileria annulata. The deduced amino acid sequence of 709 residues predicts that p67 is a membrane protein and that it lacks tandemly repeated sequences. Recombinant p67 has been expressed in Escherichia coli as a fusion protein with Sj-26, a glutathione-S-transferase of Schistosoma japonicum. Antibodies to purified recombinant proteins containing residues 9-316 or 397-709 of p67 bind to p67 in immunoblots and neutralise sporozoite infectivity in vitro. Recombinant p67 is, therefore, a candidate antigen for development of an anti-sporozoite vaccine for East Coast fever in cattle. © 1992.
Authors & Co-Authors
Nene, Vishvanath M.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Iams, Keith Paul
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Gobright, Elke I.
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Musoke, Anthony Jim Tony
Kenya, Nairobi
International Laboratory for Research on Animal Diseases, Kenya
Statistics
Citations: 81
Authors: 4
Affiliations: 1
Identifiers
Doi:
10.1016/0166-6851(92)90196-Q
ISSN:
01666851
Research Areas
Genetics And Genomics