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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Evidence against a role for SV40 in human mesothelioma
Cancer Research, Volume 65, No. 7, Year 2005
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Description
SV40 has been implicated in the etiology of 40% to 60% of human mesotheliomas. These studies could have important medical implications concerning possible sources of human infection and potential therapies if human tumors are induced by this agent. We did PCR-based analysis to detect SV40 large T antigen DNA in human mesotheliomas. None of 69 tumors in which a single copy gene was readily amplified contained detectable SV40 large T antigen sequences. Under these conditions, it was possible to detect one copy of integrated SV40 DNA per cell in a mixture containing a 5,000-fold excess of normal cells using formalin-fixed preparations. Kidney, a known reservoir of SV40 in monkeys, from some of these individuals were also negative for SV40 large T antigen sequences. A subset of mesotheliomas was analyzed for SV40 large T antigen expression by immunostaining with a highly specific SV40 antibody. These tumors as well as several human mesothelioma cell lines previously reported to contain SV40 large T antigen were negative for detection of the virally encoded oncoprotein. Moreover, mesothelioma cell lines with wild-type p53 showed normal p53 function in response to genotoxic stress, findings inconsistent with p53 inactivation by the putative presence of SV40 large T antigen. Taken together, these findings strongly argue against a role of SV40 by any known transformation mechanism in the etiology of the majority of human malignant mesotheliomas. ©2005 American Association for Cancer Research.
Authors & Co-Authors
Manfredi, James J.
United States, New York
Icahn School of Medicine at Mount Sinai
Dong, Jianli
United States, New York
Icahn School of Medicine at Mount Sinai
Liu, Wenjun
United States, New York
Icahn School of Medicine at Mount Sinai
Resnick-Silverman, Lois A.
United States, New York
Icahn School of Medicine at Mount Sinai
Qiao, Rui
United States, New York
Icahn School of Medicine at Mount Sinai
Chahinian, Philippe
United States, New York
Icahn School of Medicine at Mount Sinai
Saric, Marko
Croatia, Zagreb
Institut za Medicinska Istrazivanja I Medicinu Rada
Gibbs, Allen R.
United Kingdom, Llandough
University Hospital Llandough
Phillips, James Ian
South Africa, Johannesburg
University of the Witwatersrand
Murray, Jill F.
South Africa, Johannesburg
University of the Witwatersrand
Axten, Charles W.
United States, New York
The Graduate Center
Nolan, Robert P.
United States, New York
The Graduate Center
Aaronson, Stuart A.
United States, New York
Icahn School of Medicine at Mount Sinai
United States, New York
The Mount Sinai Medical Center
Statistics
Citations: 95
Authors: 13
Affiliations: 6
Identifiers
Doi:
10.1158/0008-5472.CAN-04-2461
ISSN:
00085472
Research Areas
Cancer
Genetics And Genomics