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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Genotypic and phenotypic analysis of HIV type 1 primary isolates from Western Cameroon
AIDS Research and Human Retroviruses, Volume 18, No. 1, Year 2002
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Description
In this study we report the molecular and biological characteristics of 19 HIV-1 primary isolates obtained in April 1999 from 47 HIV-1-infected individuals living mainly in western Cameroon. Discontinuous portions of gag, pol, and env were amplified by polymerase chain reaction and directly sequenced. Phylogenetic analysis of these sequences showed that all were of HIV-1 group M with the following genotypes: Agag/Apol/Aenv(n = 4), Agag/AGpol/AGenv(n = 2), AGgag/Apol/AGenv(n = 1), AGgag/Upol/AGenv(n = 1), AGgag/AGpol/AGenv(n = 6), Ggag/Gpol/Genv(n = 3), F2gag/F2pol/F2env(n = 1), and a novel Agag/Jpro/rt/Aint/Uenvcomplex recombinant (n = 1). This A/J/U recombinant shared the same gag-pol cross-over point with known CRF02.AG viruses and 99CMBD6, an AG recombinant from our panel of isolates. The biological phenotype of most of the isolates correlated with the clinical status of the patient. Six isolates were syncytium inducing (SI) on MT-2 cells whereas 13 isolates were of the non-syncytium-inducing phenotype (NSI). Coreceptor usage by these isolates determined on GHOST cells correlated with their biological phenotype, as all SI isolates used CXCR4 and all NSI isolates used CCR5. Our results show a high predominance of subtype A (mainly CRF02.AG-like viruses) in western Cameroon and fewer HIV-1 subtypes compared with other parts of Cameroon. Genetic variability was, however, not reflected in the biological characteristics of the isolates. The presence of a novel A/J/U complex recombinant from this region further emphasizes the role of recombination in the global evolution of HIV. © 2002 Mary Ann Liebert, Inc.
Authors & Co-Authors
Tebit, Denis Manga
Germany, Hamburg
Heinrich-pette-institut Leibniz-institut Für Experimentelle Virologie
Germany, Heidelberg
Universität Heidelberg
Zekeng, Leópold
Cameroon, Yaounde
University Teaching Hospital Yaounde
KaptuÝ, Lazare
Cameroon, Yaounde
University Teaching Hospital Yaounde
Salminen, Mika O.
Finland, Helsinki
Terveyden ja Hyvinvoinnin Laitos
Kraüsslich, Hans Georg
Germany, Hamburg
Heinrich-pette-institut Leibniz-institut Für Experimentelle Virologie
Germany, Heidelberg
Universität Heidelberg
Herchenröder, Ottmar
Germany, Hamburg
Heinrich-pette-institut Leibniz-institut Für Experimentelle Virologie
Germany, Dresden
Medizinische Fakultät Carl Gustav Carus
Statistics
Citations: 58
Authors: 6
Affiliations: 5
Identifiers
Doi:
10.1089/088922202753394709
ISSN:
08892229
Research Areas
Genetics And Genomics
Health System And Policy
Infectious Diseases
Study Locations
Cameroon