Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Simultaneous isolation of cytoplasmic endoribonuclease and exoribonuclease of Trypanosoma brucei
Molecular and Biochemical Parasitology, Volume 15, No. 1, Year 1985
Notification
URL copied to clipboard!
Description
An endoribonuclease and an exoribonuclease have been isolated simultaneously from the cytoplasm of Trypanosoma brucei by hydroxyapatite column chromatography. The endoribonuclease produced oligonucleotides from poly(adenylic acid) with 5′-phosphate and 3′-OH termini. The exoribonuclease produced only ribonucleoside 5′-phosphates from poly(adenylic acid). The relative rates of degradation of synthetic homopolynucleotides by the endoribonuclease under standard conditions were in the order poly(adenylic acid) > poly(uridylic acid) {geometrically equal to} poly(cytidylic acid); for the exoribonuclease the order was poly(adenylic acid) {geometrically equal to} poly(uridylic acid) > poly(cytidylic acid). Natural transfer and ribosomal RNAs were also degraded by both enzymes, while DNA was resistant to them. The optimal pH of activity for each enzyme was 7.5-8.0. Both ribonucleases require Ca2+ for maximum enzymatic activity. © 1985.
Authors & Co-Authors
Gbenle, George Olabode
Nigeria, Lagos
University of Lagos
Belgium, Brussels
International Institute of Cellular and Molecular Pathology
Statistics
Citations: 6
Authors: 1
Affiliations: 2
Identifiers
Doi:
10.1016/0166-6851(85)90027-1
ISSN:
01666851
Research Areas
Genetics And Genomics