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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Excretory-secretory products from adult helminth Nippostrongylus brasiliensis have in vitro bactericidal activity
Journal of Medical Microbiology, Volume 72, No. 11, Article 001762, Year 2023
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Description
Introduction. Intestinal helminths and microbiota share the same anatomical niche during infection and are likely to interact either directly or indirectly. Whether intestinal helminths employ bactericidal strategies that influence their microbial environment is not completely understood. Hypothesis. In the present study, the hypothesis that the adult hookworm Nippostrongylus brasiliensis produces molecules that impair bacterial growth in vitro, is tested. Aim. To investigate the in vitro bactericidal activity of Nippostrongylus brasiliensis against commensal and pathogenic bacteria. Methodology. The bactericidal effect of somatic extract and excretory-secretory products of adult Nippostrongylus brasiliensis on Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli, Salmonella enterica serovar Typhimurium, and Klebsiella pneumoniae) bacteria was assessed using growth assays. Minimum inhibitory concentration and minimum bactericidal concentration assays were performed using excretory-secretory products released from the pathogen. Results. Broad-spectrum in vitro bactericidal activity in excretory-secretory products, but not somatic extract of adult Nippostrongylus brasiliensis was detected. The bactericidal activity of excretory-secretory products was concentration-dependent, maintained after heat treatment, and preserved after repeated freezing and thawing. Conclusion. The results of this study demonstrate that helminths such as Nippostrongylus brasiliensis release molecules via their excretory-secretory pathway that have broad-spectrum bactericidal activity. The mechanisms responsible for this bactericidal activity remain to be determined and further studies aimed at isolating and identifying active bactericidal molecules are needed. © 2023 The Authors.
Authors & Co-Authors
Mkhize-Kwitshana, Zilungile L.
South Africa, Durban
University of Kwazulu-natal
South Africa, Tygerberg
South African Medical Research Council
Horsnell, William Gordon Charles
South Africa, Cape Town
University of Cape Town
United Kingdom, London
Medical Research Council
Icke, Christopher
United Kingdom, Birmingham
University of Birmingham
Henderson, Ian R.
Australia, Brisbane
The University of Queensland
Selkirk, Murray E.
United Kingdom, London
Imperial College London
Berkachy, Rita
United Kingdom, London
Imperial College London
Naidoo, Pragalathan
South Africa, Durban
University of Kwazulu-natal
South Africa, Tygerberg
South African Medical Research Council
Niehaus, Abraham J.
South Africa, Cape Town
Ampath Laboratories
Singh, Ravesh
South Africa, Durban
University of Kwazulu-natal
Cunningham, Adam F.
United Kingdom, Birmingham
University of Birmingham
O'Shea, Matthew K.
United Kingdom, Birmingham
University of Birmingham
Statistics
Authors: 11
Affiliations: 9
Identifiers
Doi:
10.1099/jmm.0.001762
ISSN:
00222615
Research Areas
Infectious Diseases