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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Characterization of two minicircular plasmid-like DNAs isolated from date-palm mitochondria
Current Genetics, Volume 26, No. 5-6, Year 1994
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Description
We report here the identification and characterization of two minicircular plasmid-like DNAs isolated from mitochondria of a moroccan date-palm variety. Both molecules were cloned and used as probes in Southern analyses of mitochondrial and total-cellular DNA. Evidence was obtained that these plasmid-like DNAs crosshybridized but did not show any homology to nuclear, chloroplastic, or main mitochondrial genomes. Sequence analysis revealed that both minicircles, 1 346- and 1 160-bp long, share several stretches of homology, the most important consisting of three identical clusters of lengths 42, 47 and 38 bp. In contrast, no major homology was observed with the other higher-plant plasmid-like DNAs reported so far. Sequence analysis also revealed the presence, in the same strand of one of the minicircles, of two open reading frames potentially encoding proteins 89 and 86 amino acids in length. Interestingly, Northern analyses, using single strands of each minicircle as probes, showed the presence of two transcripts hybridizing only with the strand bearing these two open reading frames. However, computer-assisted comparison of the predicted polypeptide sequences with a protein-sequence library failed to detect any significant homology to known sequences. © 1994 Springer-Verlag.
Authors & Co-Authors
Benslimane, Abdel Ali
Morocco, Marakech
Faculté Des Sciences Semlalia
Rode, André
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Quétier, Francis
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Hartmann, C.
France, Gif-sur-yvette
Institut Des Sciences Des Plantes de Paris-saclay
Statistics
Citations: 21
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1007/BF00309946
ISSN:
01728083
e-ISSN:
14320983
Research Areas
Cancer
Genetics And Genomics