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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Control of glycerol biosynthesis under high salt stress in arabidopsis
Functional Plant Biology, Volume 41, No. 1, Year 2014
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Description
Loss-of-function and gain-of-function approaches were utilised to detect the physiological importance of glycerol biosynthesis during salt stress and the role of glycerol in conferring salt tolerance in Arabidopsis. The salt stress experiment involved wild type (WT) and transgenic Arabidopsis overexpressing the yeast GPD1 gene (analogue of Arabidopsis GLY1 gene). The experiment also involved the Arabidopsis T-DNA insertion mutants gly1 (for suppression of glycerol 3-phosphate dehydrogenase or G3PDH), gli1 (for suppression of glycerol kinase or GK), and act1 (for suppression of G3P acyltransferase or GPAT). We evaluated salt tolerance levels, in conjunction with glycerol and glycerol 3-phosphate (G3P) levels and activities of six enzymes (G3PDH, ADH (alcohol dehydrogenase), ALDH (aldehyde dehydrogenase), GK, G3PP (G3P phosphatase) and GLYDH (glycerol dehydrogenase)) involved in the glycerol pathway. The GPD1 gene was used to overexpress G3PDH, a cytosolic NAD+-dependent key enzyme of cellular glycerol biosynthesis essential for growth of cells under abiotic stresses. T2 GPD1-transgenic plants and those of the two mutants gli1 and act1 showed enhanced salt tolerance during different growth stages as compared with the WT and gly1 mutant plants. These results indicate that the participation of glycerol, rather than G3P, in salt tolerance in Arabidopsis. The results also indicate that the gradual increase in glycerol levels in T2 GPD1-transgenic, and gli1 and act1 mutant plants as NaCl level increases whereas they dropped at 200mM NaCl. However, the activities of the G3PDH, GK, G3PP and GLYDH at 150 and 200mM NaCl were not significantly different. We hypothesise that mechanism(s) of glycerol retention/efflux in the cell are affected at 200mM NaCl in Arabidopsis.Journal compilation © CSIRO 2014.
Authors & Co-Authors
Bahieldin, Ahmed M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Cairo
College of Agriculture
Sabir, Jamal S.M.
Saudi Arabia, Jeddah
King Abdulaziz University
Ramadan, Ahmed M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
Agricultural Genetic Engineering Research Institute Agerl
Alzohairy, Ahmed Mansour
Egypt, Zagazig
Faculty of Agriculture
Younis, Rania A.
Egypt, Cairo
College of Agriculture
Shokry, Ahmed M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
Agricultural Genetic Engineering Research Institute Agerl
Gadalla, Nour O.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
National Research Centre
Edris, S. A.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Cairo
College of Agriculture
Hassan, Sabah M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Cairo
College of Agriculture
Al-Kordy, Magdy A.A.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Giza
National Research Centre
Kamal, Khalid B.H.
Saudi Arabia, Jeddah
King Abdulaziz University
Rabah, Samar Omar A.
Saudi Arabia, Jeddah
King Abdulaziz University
Abuzinadah, Osama A.
Saudi Arabia, Jeddah
King Abdulaziz University
El-Domyati, Fotouh M.
Saudi Arabia, Jeddah
King Abdulaziz University
Egypt, Cairo
College of Agriculture
Statistics
Citations: 15
Authors: 14
Affiliations: 5
Identifiers
Doi:
10.1071/FP13005
ISSN:
14454408
Research Areas
Genetics And Genomics
Substance Abuse
Study Approach
Quantitative