Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Separation and determination of clopidogrel and its impurities by capillary electrophoresis
Journal of Pharmaceutical and Biomedical Analysis, Volume 49, No. 2, Year 2009
Notification
URL copied to clipboard!
Description
Clopidogrel bisulphate, an anti-platelet drug, has been separated from its impurities, namely impurity A, B and C by capillary zone electrophoresis (CZE) using uncoated fused-silica capillary (50.0 μm internal diameter, 31.2 cm total length). Four factors affected the separation: buffer concentration, pH of the buffer, concentration of the chiral selector and the applied voltage. Optimization and robustness studies were performed with the aid of reduced central composite experimental design. The buffer used was triethylamine-phosphoric acid and the chosen chiral selector was sulphated β-cyclodextrin (SCD). The best separation was achieved by using 10 mM buffer, pH 2.3, containing 5% (mass/volume (m/v)) SCD. Reversed polarity mode was used with an applied voltage of -12 kV and the capillary temperature was maintained at 20 °C. The method was validated for quantitative determination of the drug. It offered a limit of detection (LOD) of 0.13 μg/ml, a limit of quantitation (LOQ) of 0.4 μg/ml, and a linearity range of 0.4-300 μg/ml. Commercial bulk samples were analyzed using the developed method. © 2008 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Fayed, Ahmed Salah
Belgium, Leuven
Departement Farmaceutische en Farmacologische Wetenschappen
Egypt, Cairo
Faculty of Pharmacy
Weshahy, Soheir Abd El Fatah
Egypt, Cairo
Faculty of Pharmacy
Shehata, Mostafa Abdelaty M.
Egypt, Cairo
Faculty of Pharmacy
Hassan, Nagiba Y.M.
Egypt, Cairo
Faculty of Pharmacy
Pauwels, Jochen
Belgium, Leuven
Departement Farmaceutische en Farmacologische Wetenschappen
Hoogmartens, Joseph
Belgium, Leuven
Departement Farmaceutische en Farmacologische Wetenschappen
van Schepdael, Ann
Belgium, Leuven
Departement Farmaceutische en Farmacologische Wetenschappen
Statistics
Citations: 53
Authors: 7
Affiliations: 2
Identifiers
Doi:
10.1016/j.jpba.2008.10.031
ISSN:
07317085
Study Approach
Quantitative