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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Classification of sharks in the egyptian mediterranean waters using morphological and DNA barcoding approaches
PLoS ONE, Volume 6, No. 11, Article e27001, Year 2011
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Description
The identification of species constitutes the first basic step in phylogenetic studies, biodiversity monitoring and conservation. DNA barcoding, i.e. the sequencing of a short standardized region of DNA, has been proposed as a new tool for animal species identification. The present study provides an update on the composition of shark in the Egyptian Mediterranean waters off Alexandria, since the latest study to date was performed 30 years ago, DNA barcoding was used in addition to classical taxonomical methodologies. Thus, 51 specimen were DNA barcoded for a 667 bp region of the mitochondrial COI gene. Although DNA barcoding aims at developing species identification systems, some phylogenetic signals were apparent in the data. In the neighbor-joining tree, 8 major clusters were apparent, each of them containing individuals belonging to the same species, and most with 100% bootstrap value. This study is the first to our knowledge to use DNA barcoding of the mitochondrial COI gene in order to confirm the presence of species Squalus acanthias, Oxynotus centrina, Squatina squatina, Scyliorhinus canicula, Scyliorhinus stellaris, Mustelus mustelus, Mustelus punctulatus and Carcharhinus altimus in the Egyptian Mediterranean waters. Finally, our study is the starting point of a new barcoding database concerning shark composition in the Egyptian Mediterranean waters (Barcoding of Egyptian Mediterranean Sharks [BEMS], http://www.boldsystems.org/views/projectlist.php?Barcoding%20Fish%20%28FishBOL%29. © 2011 Moftah et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3206905/bin/pone.0027001.s001.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3206905/bin/pone.0027001.s002.pdf
Authors & Co-Authors
Moftah, Marie Z.
Egypt, Alexandria
Faculty of Science
Aziz, Sayeda H.Abdel
Egypt, Alexandria
Faculty of Science
El Ramah, Sara
Egypt, Alexandria
Faculty of Science
France, Bordeaux
Université de Bordeaux
France, Bordeaux
Institut Interdisciplinaire de Neurosciences
Favereaux, Alexandre
France, Bordeaux
Université de Bordeaux
France, Bordeaux
Institut Interdisciplinaire de Neurosciences
Statistics
Citations: 37
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1371/journal.pone.0027001
e-ISSN:
19326203
Research Areas
Genetics And Genomics