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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Haploinsufficiency of Pkd2 is associated with increased tubular cell proliferation and interstitial fibrosis in two murine Pkd2 models
Nephrology Dialysis Transplantation, Volume 21, No. 8, Year 2006
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Description
Background. Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited human kidney disease and is caused by germline mutations in PKD1 (85%) or PKD2 (15%) It has been estimated that around 1% of tubular cells give rise to cysts, and cell hyperproliferation has been noted to be a cardinal feature of cystic epithelium. Nevertheless, it is uncertain whether the increase in proliferative index observed is an early or late feature of the cystic ADPKD kidney. Methods. Two Pkd2 mouse mutants (WS25 and WS183) have been recently generated as orthologous models of PKD2. To determine the effect of Pkd2 dosage on cell proliferation, cyst formation and renal fibrosis, we studied renal tissue from Pkd2WS25/WS25 and Pkd2+/- mice by histological analysis. We also examined the proliferative index in archival nephrectomy tissue obtained from patients with ADPKD and normal controls. Results. The proliferative index of non-cystic tubules in Pkd2 mutant mice as assessed by proliferating cell nuclear antigen and Ki67-positive nuclei was between 1-2%, values 5-10 times higher than control tissue. Similarly, the proliferative index of non-cystic tubules in human ADPKD kidneys was 40 times higher than corresponding controls. In Pkd2 mutant mice, significant correlations were found between the fibrosis score and the mean cyst area as well as with the proliferative index. Of significance, proliferating tubular cells were uniformly positive for polycystin-2 expression in Pkd2+/-kidney. Conclusion. These results suggest that an increase in cell proliferation is an early event preceding cyst formation and can result from haploinsufficiency at Pkd2. The possible pathogenic link between tubular cell proliferation, interstitial fibrosis and cyst formation is discussed. © 2006 Oxford University Press.
Authors & Co-Authors
Chang, Ming Yang
United Kingdom, Sheffield
The University of Sheffield
Parker, Emma
United Kingdom, Sheffield
The University of Sheffield
Ibrahim, Salwa K.
Egypt, Giza
Cairo University
Shortland, John R.
United Kingdom, Sheffield
Sheffield Teaching Hospitals Nhs Foundation Trust
El-Nahas, Meguid A.
United Kingdom, Sheffield
The University of Sheffield
Haylor, John Lionel
United Kingdom, Sheffield
The University of Sheffield
Ong, Albert C.M.
United Kingdom, Sheffield
The University of Sheffield
Statistics
Citations: 78
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1093/ndt/gfl150
ISSN:
09310509
e-ISSN:
14602385
Research Areas
Cancer
Noncommunicable Diseases