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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
A steady state of CD4
+
T cell memory maturation and activation is established during primary subtype C HIV-1 infection
Journal of Immunology, Volume 184, No. 9, Year 2010
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Description
The functional integrity of CD4+ T cells is crucial for well-orchestrated immunity and control of HIV-1 infection, but their selective depletion during infection creates a paradox for understanding a protective response. We used multiparameter flow cytometry to measure activation, memory maturation, and multiple functions of total and Ag-specific CD4+ T cells in 14 HIV-1- and CMV-coinfected individuals at 3 and 12 mo post HIV-1 infection. Primary HIV-1 infection was characterized by elevated levels of CD38, HLA-DR, and Ki67 in total memory and Gag-specific CD4+ and CD8 + T cells. In both HIV-infected and 15 uninfected controls, the frequency of activated cells was uniformly distributed among early differentiated (ED; CD45RO+CD27+), late differentiated (CD45RO+CD27-), and fully differentiated effector (CD45RO-CD27-) memory CD4+ T cells. In HIV-1-infected individuals, activated CD4+ T cells significantly correlated with viremia at 3 mo postinfection (r = 0.79, p = 0.0007) and also harbored more gag provirus DNA copies than nonactivated cells (p = 0.04). Moreover, Gag-specific ED CD4+ T cells inversely associated with plasma viral load (r = 20.87, p < 0.0001). Overall, we show that low copy numbers of gag provirus and plasma RNA copies associated with low CD4 activation as well as accumulation of ED HIV-specific CD4+ memory. Significant positive correlations between 3 and 12 mo activation and memory events highlighted that a steady state of CD4+ T cell activation and memory maturation was established during primary infection and that these cells were unlikely to be involved in influencing the course of viremia in the first 12 mo of HIV-1 infection. Copyright © 2010 by The American Association of Immunologists, Inc.
Authors & Co-Authors
Maenetje, Pholo Wilson
South Africa, Johannesburg
National Institute for Communicable Diseases
Riou, Catherine
South Africa, Johannesburg
National Institute for Communicable Diseases
Casazza, Joseph P.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Ambrozak, David R.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Hill, Brenna J.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
Gray, Glenda E.
South Africa, Johannesburg
University of the Witwatersrand
Koup, Richard A.
United States, Bethesda
National Institute of Allergy and Infectious Diseases Niaid
De Bruyn, Guy
South Africa, Johannesburg
University of the Witwatersrand
Gray, Clive M.
South Africa, Johannesburg
National Institute for Communicable Diseases
Statistics
Citations: 24
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.4049/jimmunol.0903771
ISSN:
00221767
e-ISSN:
15506606
Research Areas
Genetics And Genomics
Infectious Diseases