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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Imipenem resistance in Klebsiella pneumoniae is associated to the combination of plasmid-mediated CMY-4 AmpC β-Lactamase and loss of an outer membrane protein
Microbial Drug Resistance, Volume 18, No. 5, Year 2012
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Description
This study was conducted to identify the molecular mechanisms of imipenem resistance in a Klebsiella pneumoniae (Kp16137) isolate recovered in August 2008 at the University Hospital Sahloul, Sousse, Tunisia. The strain was identified with the API 20E system; antibiotic-containing disks were used for detection of antibiotic susceptibility by a disk diffusion assay. We investigated the presence of β-lactamases by PCR, using specific primers for blaTEM, blaSHV, blaCTX-M, blaOXA, blaCMY, blaACC, blaFOX, blaIMP, blaKPC, blaVIM, and by sequencing. Extraction of plasmid DNA from Kp16137 and the transconjugant was performed by the method of Kado. Southern transfer was performed on nylon. The membrane was hybridized with a specific probe for the blaCMY-2 gene. Outer membrane proteins were isolated and were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis on 12% polyacrylamide gel. K. pneumoniae Kp16137 was resistant to all available β-lactams, including third generation cephalosporins and carbapenems. The screening of β-lactamases showed the presence of three β-lactamases: TEM-1, SHV-61, and CMY-4. The CMY-4 β-lactamase was located on an 80-kb plasmid. An analysis of the outer membrane proteins of this isolate revealed that it lacked a porin of 42 kDa. The loss of this outer membrane protein band correlated with imipenem resistance in this strain. In K. pneumoniae 16137, synthesis of a plasmid-mediated β-lactamase: AmpC CMY-4, together with alteration in permeability led to resistance to all available β-lactams and carbapenems. © Mary Ann Liebert, Inc.
Authors & Co-Authors
Dahmen, Safia
Tunisia, Sousse
University Hospital of Sahloul
France, Paris
Sorbonne Université
Mansour, Wejdène
Tunisia, Sousse
University Hospital of Sahloul
Charfi, Karama
Tunisia, Sousse
University Hospital of Sahloul
Boujâafar, Noureddine
Tunisia, Sousse
University Hospital of Sahloul
Arlet, Guillaume A.
France, Paris
Sorbonne Université
Bouallègue, Olfa
Tunisia, Sousse
University Hospital of Sahloul
Statistics
Citations: 28
Authors: 6
Affiliations: 2
Identifiers
Doi:
10.1089/mdr.2011.0214
ISSN:
10766294
e-ISSN:
19318448
Research Areas
Genetics And Genomics
Health System And Policy
Study Locations
Tunisia