Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Variable recombination dynamics during the emergence, transmission and 'disarming' of a multidrug-resistant pneumococcal clone
BMC Biology, Volume 12, Article 49, Year 2014
Notification
URL copied to clipboard!
Description
Background: Pneumococcal β-lactam resistance was first detected in Iceland in the late 1980s, and subsequently peaked at almost 25% of clinical isolates in the mid-1990s largely due to the spread of the internationally-disseminated multidrug-resistant PMEN2 (or Spain6B-2) clone of Streptococcus pneumoniae.Results: Whole genome sequencing of an international collection of 189 isolates estimated that PMEN2 emerged around the late 1960s, developing resistance through multiple homologous recombinations and the acquisition of a Tn5253-type integrative and conjugative element (ICE). Two distinct clades entered Iceland in the 1980s, one of which had acquired a macrolide resistance cassette and was estimated to have risen sharply in its prevalence by coalescent analysis. Transmission within the island appeared to mainly emanate from Reykjavík and the Southern Peninsular, with evolution of the bacteria effectively clonal, mainly due to a prophage disrupting a gene necessary for genetic transformation in many isolates. A subsequent decline in PMEN2's prevalence in Iceland coincided with a nationwide campaign that reduced dispensing of antibiotics to children in an attempt to limit its spread. Specific mutations causing inactivation or loss of ICE-borne resistance genes were identified from the genome sequences of isolates that reverted to drug susceptible phenotypes around this time. Phylogenetic analysis revealed some of these occurred on multiple occasions in parallel, suggesting they may have been at least temporarily advantageous. However, alteration of 'core' sequences associated with resistance was precluded by the absence of any substantial homologous recombination events.Conclusions: PMEN2's clonal evolution was successful over the short-term in a limited geographical region, but its inability to alter major antigens or 'core' gene sequences associated with resistance may have prevented persistence over longer timespans. © 2014 Croucher et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S1.xlsx
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S2.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S3.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S4.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S5.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S6.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S7.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S8.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S9.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S10.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S11.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S12.docx
https://efashare.b-cdn.net/share/pmc/articles/PMC4094930/bin/1741-7007-12-49-S13.docx
Authors & Co-Authors
Croucher, Nicholas J.
Unknown Affiliation
Hanage, William Paul
Unknown Affiliation
Harris, Simon R.
Unknown Affiliation
McGee, Lesley
Unknown Affiliation
van der Linden, Mark P.G.
Unknown Affiliation
de Lencastre, Hermínia M.
Unknown Affiliation
Sa-Leao, R.
Unknown Affiliation
Song, Jae-hoon
Unknown Affiliation
Ko, Kwan Soo
Unknown Affiliation
Beall, Bernard W.
Unknown Affiliation
Klugman, K. P.
Unknown Affiliation
Parkhill, Julian
Unknown Affiliation
Tomasz, A.
Unknown Affiliation
Kristinsson, Karl Gustaf
Unknown Affiliation
Bentley, Stephen D.
Unknown Affiliation
Statistics
Citations: 15
Authors: 15
Affiliations: 16
Identifiers
Doi:
10.1186/1741-7007-12-49
e-ISSN:
17417007
Research Areas
Genetics And Genomics
Maternal And Child Health
Study Design
Cross Sectional Study