Antioxidant and anticholinesterase activity of essential oils and ethanol extracts of Thymus algeriensis and Teucrium polium from Algeria

This study aimed to investigate the cholinesterase (ChE) inhibitory and antioxidant activities of essential oil (EO) and ethanolic extract (EE) of two Lamiaceae medicinal plants from Algeria: Thymus algeriensis Boiss. & Reut. and Teucrieum polium subsp capitatum. The chemical composition of EOs analyzed by GC and GC–MS revealed the presence of carvacrol (43.2%), p-cymene (18.7%) and γ-terpinene (14.8%) as major compounds in T. algeriensis, while germacrene D (25.0%), bicyclogermacrene (10.4%), β-pinene (11.3%) and spathulenol (5.8%) were the most important components in T. polium oil. The best inhibitory activity on acetylcholinesterase (AChE) was exhibited by the EO of T. algeriensis. On the other hand, T. polium oil was more efficient against butyrylcholinesterase (BChE) than against AChE, whereas EEs showed weak or no inhibitory effect, particularly against AChE. Antioxidant activity was evaluated by β-carotene bleaching, ferric and cupric reducing powers and inhibition of ABTS•+, DPPH• and superoxide radicals. EOs showed different antioxidant trends depending on the assay used while ethanol extracts had high activity with all tests.