Development and validation of an in vitro resazurin-based susceptibility assay against madurella mycetomatis
Antimicrobial Agents and Chemotherapy, Volume 65, No. 3, Article e01338-20, Year 2021
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We present an in vitro susceptibility assay for Madurella mycetomatis hyphae using resazurin for endpoint reading. Using this assay, reproducible MICs were obtained for amphotericin B, itraconazole, voriconazole, posaconazole, terbinafine, and micafungin. Results were comparable with those of a 2,3-bis-(2-methoxy-4- nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT)-based susceptibility assay. The lowest MICs were obtained for itraconazole and posaconazole (MIC50, 0.016mg/ml) followed by voriconazole (MIC50, 0.063mg/ml). Amphotericin B, micafungin, and terbinafine appeared much less effective.