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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Molecular Epidemiology, Drug Susceptibility and Economic Aspects of Tuberculosis in Mubende District, Uganda
PLoS ONE, Volume 8, No. 5, Article e64745, Year 2013
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Description
Background:Tuberculosis (TB) remains a global public health problem whose effects have major impact in developing countries like Uganda. This study aimed at investigating genotypic characteristics and drug resistance profiles of Mycobacterium tuberculosis isolated from suspected TB patients. Furthermore, risk factors and economic burdens that could affect the current control strategies were studied.Methods:TB suspected patients were examined in a cross-sectional study at the Mubende regional referral hospital between February and July 2011. A questionnaire was administered to each patient to obtain information associated with TB prevalence. Isolates of M. tuberculosis recovered during sampling were examined for drug resistance to first line anti-TB drugs using the BACTEC-MGIT960TMsystem. All isolates were further characterized using deletion analysis, spoligotyping and MIRU-VNTR analysis. Data were analyzed using different software; MIRU-VNTR plus, SITVITWEB, BioNumerics and multivariable regression models.Results:M. tuberculosis was isolated from 74 out of 344 patients, 48 of these were co-infected with HIV. Results from the questionnaire showed that previously treated TB, co-infection with HIV, cigarette smoking, and overcrowding were risk factors associated with TB, while high medical related transport bills were identified as an economic burden. Out of the 67 isolates that gave interpretable results, 23 different spoligopatterns were detected, nine of which were novel patterns. T2 with the sub types Uganda-I and Uganda-II was the most predominant lineage detected. Antibiotic resistance was detected in 19% and multidrug resistance was detected in 3% of the isolates.Conclusion:The study detected M. tuberculosis from 21% of examined TB patients, 62% of whom were also HIV positive. There is a heterogeneous pool of genotypes that circulate in this area, with the T2 lineage being the most predominant. High medical related transport bills and drug resistance could undermine the usefulness of the current TB strategic interventions. © 2013 Muwonge et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3669366/bin/pone.0064745.s001.pdf
Authors & Co-Authors
Muwonge, Adrian
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
United Kingdom, Midlothian
University of Edinburgh, Roslin Institute
Malama, Sydney K.
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Zambia, Lusaka
Evelyn Hone College
Johansen, Tone Bjordal
Norway, Oslo
Veterinærinstituttet
Kankya, Clovice
Uganda, Kampala
Makerere University
Biffa, Demelash
United States, Tucson
The University of Arizona
Ssengooba, Willy
Uganda, Kampala
Makerere University College of Health Sciences
Godfroid, Jacques
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
DjØnne, Berit Kristoffersen
Norway, Oslo
Veterinærinstituttet
Skjerve, Eystein
Norway, As
Norges Miljø- og Biovitenskapelige Universitet
Statistics
Citations: 29
Authors: 9
Affiliations: 7
Identifiers
Doi:
10.1371/journal.pone.0064745
e-ISSN:
19326203
Research Areas
Health System And Policy
Infectious Diseases
Substance Abuse
Study Design
Cross Sectional Study
Study Approach
Quantitative
Study Locations
Uganda