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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Determination of anthocyanins in wine by direct injection liquid chromatography-diode array detection-mass spectrometry and classification of wines using discriminant analysis
Journal of Chromatography A, Volume 1054, No. 1-2, Year 2004
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Description
A rapid HPLC-diode array detection (DAD) method was developed for the routine analysis of 16 anthocyanins in wine. Direct injection of filtered wine samples followed by selective detection at 520 nm allowed quantitation of these compounds in red wines. The method was linear for malvidin-3-glucoside over the range 5-250 ppm, and the limit of detection for this compound was 0.18 ppm. A volatile mobile phase is used, which enables hyphenation to mass spectrometry (MS). With HPLC-MS, a total of 44 pigments could be identified in South African wines. Obtained mass spectra are discussed for a series of representative wine constituents and results are compared with literature references. An attempt was made to differentiate between different cultivars according to the anthocyanin content using stepwise forward linear discriminant analysis (LDA). © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
de Villiers, André J.
South Africa, Stellenbosch
Stellenbosch University
Vanhoenacker, Gerd
Belgium, Ghent
Universiteit Gent
Májek, Pavel
Slovakia, Bratislava
Slovak University of Technology in Bratislava
Sandra, Pat J.F.
South Africa, Stellenbosch
Stellenbosch University
Belgium, Ghent
Universiteit Gent
Statistics
Citations: 122
Authors: 4
Affiliations: 3
Identifiers
Doi:
10.1016/j.chroma.2004.07.087
ISSN:
00219673