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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
C57BL/6 and congenic interleukin-10-deficient mice can serve as models of Campylobacter jejuni colonization and enteritis
Infection and Immunity, Volume 75, No. 3, Year 2007
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Description
Campylobacter jejuni is a globally distributed cause of human food-borne enteritis and has been linked to chronic joint and neurological diseases. We hypothesized that C. jejuni 11168 colonizes the gastrointestinal tract of both C57BL/6 mice and congenic C57BL/6 interleukm-10-deficient (IL-10-/-) mice and that C57BL/6 IL-10-/- mice experience C. jejuni 11168-mediated clinical signs and pathology. Individually housed mice were challenged orally with C. jejuni 11168, and the course of infection was monitored by clinical examination, bacterial culture, C. jejuni-specific PCR, gross pathology, histopathology, immunohistochemistry, and anti-C. jejuni-specific serology. Ceca of C. jejuni 11168-infected mice were colonized at high rates: ceca of 50/50 wild-type mice and 168/170 DL-10-1- mice were colonized. In a range from 2 to 35 days after infection with C. jejuni 11168, C57BL/6 IL-10-7- mice developed severe typhlocolitis best evaluated at the ileocecocolic junction. Rates of colonization and enteritis did not differ between male and female mice. A dose-response experiment showed that as little as 106 CFU produced significant disease and pathological lesions similar to responses seen in humans. Immunohistochemical staining demonstrated C. jejuni antigens within gastrointestinal tissues of infected mice. Significant anti-C. jejuni plasma immunoglobulin levels developed by day 28 after infection in both wild-type and DL-10-deficient animals; antibodies were predominantly T-helper-cell 1 (Th1)-associated subtypes. These results indicate that the colonization of the mouse gastrointestinal tract by C. jejuni 11168 is necessary but not sufficient for the development of enteritis and that C57BL/6 IL-10-/- mice can serve as models for the study of C. jejuni enteritis in humans. Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Authors & Co-Authors
Mansfield, L. S.
United States, East Lansing
Michigan State University
Bell, J. A.
United States, East Lansing
Michigan State University
Wilson, D. L.
United States, East Lansing
Michigan State University
Murphy, A. J.
United States, East Lansing
Michigan State University
Elsheikha, Hany M.
United States, East Lansing
Michigan State University
Egypt, Mansoura
Mansoura University
United Kingdom, Nottingham
University of Nottingham, Faculty of Medicine and Health Sciences
Rathinam, V. A.K.
United States, East Lansing
Michigan State University
Fierro, B. R.
United States, East Lansing
Michigan State University
Linz, John E.
United States, East Lansing
Michigan State University
Young, Vincent Bensan
United States, East Lansing
Michigan State University
Statistics
Citations: 111
Authors: 9
Affiliations: 3
Identifiers
Doi:
10.1128/IAI.00833-06
ISSN:
00199567
Research Areas
Food Security
Study Approach
Quantitative
Participants Gender
Male
Female