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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Ex vivo anti-malarial drugs sensitivity profile of Plasmodium falciparum field isolates from Burkina Faso five years after the national policy change
Malaria Journal, Volume 13, No. 1, Article 207, Year 2014
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Description
Background: The recent reports on the decreasing susceptibility of Plasmodium falciparum to artemisinin derivatives along the Thailand and Myanmar border are worrying. Indeed it may spread to India and then Africa, repeating the same pattern observed for chloroquine resistance. Therefore, it is essential to start monitoring P. falciparum sensitivity to artemisinin derivatives and its partner drugs in Africa. Efficacy of AL and ASAQ were tested by carrying out an in vivo drug efficacy test, with an ex vivo study against six anti-malarial drugs nested into it. Results of the latter are reported here. Methods. Plasmodium falciparum ex-vivo susceptibility to chloroquine (CQ), quinine (Q), lumefantrine (Lum), monodesethylamodiaquine (MDA), piperaquine (PPQ) and dihydroartemisinin (DHA) was investigated in children (6 months - 15 years) with a parasitaemia of at least ≥4,000/μl. The modified isotopic microtest technique was used. The results of cellular proliferation were analysed using ICEstimator software to determine the 50% inhibitory concentration (IC50) values. Results: DHA was the most potent among the 6 drugs tested, with IC50 values ranging from 0.8 nM to 0.9 nM (Geometric mean IC50 = 0.8 nM; 95% CI [0.8 - 0.9]). High IC50 values ranged between 0.8 nM to 166.1 nM were reported for lumefantrine (Geometric mean IC50 = 25.1 nM; 95% CI [22.4 - 28.2]). MDA and Q IC50s were significantly higher in CQ-resistant than in CQ-sensitive isolates (P = 0.0001). However, the opposite occurred for Lum and DHA (P < 0.001). No difference was observed for PPQ. Conclusion: Artemisinin derivatives are still very efficacious in Burkina Faso and DHA-PPQ seems a valuable alternative ACT. The high lumefantrine IC50 found in this study is worrying as it may indicate a decreasing efficacy of one of the first-line treatments. This should be further investigated and monitored over time with large in vivo and ex vivo studies that will include also plasma drug measurements. © 2014 Tinto et al.; licensee BioMed Central Ltd.
Authors & Co-Authors
Tinto, Halidou
Burkina Faso, Ouagadougou
Centre Muraz
Burkina Faso, Ouagadougou
Institut de Recherche en Sciences de la Santé
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Bonkian, Léa N.
Burkina Faso, Ouagadougou
Centre Muraz
Nana, Louis A.
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Yerbanga, Isidore
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Lingani, Moussa
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Kazienga, Adama
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Valea, Innocent
Burkina Faso, Ouagadougou
Centre Muraz
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Sorgho, Hermann
Burkina Faso, Ouagadougou
Institut de Recherche en Sciences de la Santé
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Kpoda, Hervé B.N.
Burkina Faso, Ouagadougou
Centre Muraz
Guiguemdé, Tinga Robert
Burkina Faso, Ouagadougou
Centre Muraz
Burkina Faso
Irss/clinical Research Unit of Nanoro Crun
Burkina Faso
Institut Supérieur Des Sciences de la Santé Inssa
Ouedraogo, Jean Bosco
Burkina Faso, Ouagadougou
Centre Muraz
Burkina Faso, Ouagadougou
Institut de Recherche en Sciences de la Santé
Mens, Pètra Francisca
Netherlands, Amsterdam
Royal Tropical Institute - Kit
Schallig, H. D. F. H.
Netherlands, Amsterdam
Royal Tropical Institute - Kit
D'Alessandro, Umberto
Gambia, Fajara
Gambia
Belgium, Antwerpen
Prins Leopold Instituut Voor Tropische Geneeskunde
Statistics
Citations: 14
Authors: 14
Affiliations: 7
Identifiers
Doi:
10.1186/1475-2875-13-207
e-ISSN:
14752875
Research Areas
Health System And Policy
Maternal And Child Health
Study Locations
Burkina Faso