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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Simultaneous detection and quantification of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in fecal samples using multiplex real-time PCR
American Journal of Tropical Medicine and Hygiene, Volume 77, No. 4, Year 2007
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Description
A multiplex real-time PCR was developed and evaluated for the simultaneous detection of Ancylostoma duodenale, Necator americanus, and Oesophagostomum bifurcum in fecal samples. Using well-defined control samples (N = 150), known positive fecal samples (N = 50), and fecal samples from an area in Ghana where human infections with all 3 nematode species are endemic (N = 339), the method proved to be highly specific and sensitive. Cycle threshold (Ct) values, reflecting parasite-specific DNA load, showed significant correlation with the intensity of infection as measured by microscopy using Kato-Katz fecal smears or by species specific third-stage larval count after coproculture. The multiplex real-time PCR described combined with the simple fecal sample collection procedure and the potential for high throughput makes this approach a powerful diagnostic tool to study species-specific transmission patterns of human hookworm-like infections. Moreover, this procedure facilitates monitoring of intervention programs and allows species-specific detection of treatment failure following rounds of mass treatment. Copyright © 2007 by The American Society of Tropical Medicine and Hygiene.
Authors & Co-Authors
Verweij, Jaco J.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Brienen, Eric A.T.
Netherlands, Leiden
Leids Universitair Medisch Centrum
Ziem, Juventus Benogle
Ghana, Tamale
University for Development Studies Ghana
Yelifari, L.
Ghana, Accra
Ministry of Health Ghana
Polderman, Anton M.
Netherlands, Leiden
Leids Universitair Medisch Centrum
van Lieshout, Lisette
Netherlands, Leiden
Leids Universitair Medisch Centrum
Statistics
Citations: 195
Authors: 6
Affiliations: 3
Identifiers
Doi:
10.4269/ajtmh.2007.77.685
ISSN:
00029637
Research Areas
Genetics And Genomics
Health System And Policy
Study Design
Randomised Control Trial
Study Locations
Ghana