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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
medicine
Single nucleotide polymorphism at exon 7 splice acceptor site of OAS1 gene determines response of hepatitis C virus patients to interferon therapy
Journal of Gastroenterology and Hepatology (Australia), Volume 26, No. 5, Year 2011
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Description
Background and Aim: Response to interferon therapy and disease progression in hepatitis C virus (HCV) infected patients differs among individuals, suggesting a possibility of a contribution of host genetic factors. 2′-5′-oligoadenylate synthetase 1 (OAS1), an important component of the innate immune system with a proven antiviral function, may therefore have a relationship with the response to interferon therapy and clinical course of HCV disease. Our aim was to determine the frequency of single nucleotide polymorphism (SNP) at exon 7 splice acceptor site (SAS) of the OAS1 gene in relation to the interferon response and status of HCV infection. Methods: A 203bp fragment containing exon 7 SAS was amplified in 70 HCV chronic patients and 50 healthy controls. SNP was examined using restriction fragment length polymorphism (RFLP) genotyping method. Correlations of SNP genotypes with response to interferon and clinical status of patients were statistically analyzed. Results: There was an increasing trend of response from AA to AG to GG genotypes (P=0.007). Genotype AA was associated with non-response to interferon and higher degree of liver fibrosis (P=0.05). Multivariate analysis showed this SNP as independent and a significant determinant of the outcome of interferon therapy (odds ratio 4.913 [95% confidence interval 1.365-8.2], P=0.006). Conclusions: This is the first study to show a significant association between the functional SNP at exon 7 SAS of OAS1 gene and the viral response to interferon in chronic HCV patients. Patients with AA genotype were associated with progressive HCV disease and viral resistance to interferon therapy. This OAS SNP is a potential bio-marker to predict IFN response in chronic hepatitis C patients. © 2011 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd.
Authors & Co-Authors
El Awady, Mostafa K.
Egypt, Giza
National Research Centre
Anany, Mohamed A.
Egypt, Giza
National Research Centre
Esmat, Gamal
Egypt, Cairo
Faculty of Medicine
Zayed, Naglaa Ali
Egypt, Cairo
Faculty of Medicine
Tabll, Ashraf Abdou
Egypt, Giza
National Research Centre
Helmy, Amr S.
Egypt, Cairo
National Liver Institute
El Zayady, Abdel Rahman
Egypt, Cairo
Cairo Liver Center
Abdalla, Mohga S.
Egypt, Helwan
Faculty of Science
Sharada, Hayat Mohamed I.
Egypt, Helwan
Faculty of Science
El-Raziky, Maissa S.
Egypt, Cairo
Faculty of Medicine
Elakel, Wafaa Ahmed
Egypt, Cairo
Faculty of Medicine
Abdalla, Shadia
Egypt, Cairo
El-sahel Teaching Hospital
Bader El Din, Noha G.
Egypt, Giza
National Research Centre
Statistics
Citations: 45
Authors: 13
Affiliations: 6
Identifiers
Doi:
10.1111/j.1440-1746.2010.06605.x
ISSN:
08159319
e-ISSN:
14401746
Research Areas
Genetics And Genomics
Infectious Diseases
Study Design
Case-Control Study