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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Comparison of serum HBsAg quantitation by four immunoassays, and relationships of HBsAg level with HBV replication and HBV genotypes
PLoS ONE, Volume 7, No. 3, Article e32143, Year 2012
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Description
Background: The decline in hepatitis B virus surface antigen (HBsAg) may be an early predictor of the viral efficacy of Hepatitis B virus (HBV) therapy. The HBsAg levels obtained by different immunoassays now need comparing and the relationships between levels of HBsAg and HBV DNA alongside HBsAg and genotype must be evaluated. Methodology/Principal Findings: HBsAg levels were compared among 80 patients using the Abbott Architect assay, a commercial immunoassay approved for HBsAg detection and quantitation, and three other assays derived from immunoassays approved for HBsAg detection (manufactured by Diasorin, Bio-Rad and Roche). Good correlation was found between the Abbot vs. Diasorin, Bio-Rad and Roche assays with narrow 95% limits of agreement and small mean differences: -0.06 to 0.11, -0.09 log 10 IU/mL; -0.57 to 0.64, -0.04 log 10 IU/mL; -0.09 to 0.45, -0.27 log 10 IU/mL, respectively. These agreements were not affected by genotypes A or D. HBsAg was weakly correlated with HBV DNA, whatever the HBsAg assay used: Abbott, ρ = 0.36 p = 0.001, Diasorin ρ = 0.34, p = 0.002; Bio-Rad ρ = 0.37, p<0.001; or Roche ρ = 0.41, p<0.001. This relationship between levels of HBsAg and HBV DNA seemed to depend on genotypes. Whereas HBsAg (Abbott assay) tended to correlate with HBV DNA for genotype A (ρ = 0.44, p = 0.02), no such correlation was significant for genotypes D (ρ = 0.29, p = 0.15). Conclusion/Significance: The quantitation of HBsAg in routine clinical samples is comparable between the reference assay and the adapted assays with acceptable accuracy limits, low levels of variability and minimum discrepancy. While HBsAg quantitation is not affected by HBV genotype, the observed association between levels of HBsAg and HBV DNA seems genotype dependent. © 2012 Tuaillon et al.
Authors & Co-Authors
Tuaillon, Édouard
France, Montpellier
Université de Montpellier
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Mondain, Anne Marie
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Nagot, Nicolas
France, Montpellier
Université de Montpellier
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
France, Montpellier
Départment de L'information Médicale
Ottomani, Laure
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Kania, Dramane
France, Montpellier
Université de Montpellier
Burkina Faso, Ouagadougou
Centre Muraz
Nogue, Erika
France, Montpellier
Départment de L'information Médicale
Rubbo, Pierre Alain
France, Montpellier
Université de Montpellier
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Pageaux, Georges Philippe
France, Paris
Inserm
van de Perre, Philippe
France, Montpellier
Université de Montpellier
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Ducos, Jacques
France, Montpellier
Université de Montpellier
France, Montpellier
Centre Hospitalier Universitaire de Montpellier
Statistics
Citations: 41
Authors: 10
Affiliations: 5
Identifiers
Doi:
10.1371/journal.pone.0032143
e-ISSN:
19326203
Research Areas
Genetics And Genomics
Infectious Diseases