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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Global sequence variation in the histidine-rich proteins 2 and 3 of Plasmodium falciparum: Implications for the performance of malaria rapid diagnostic tests
Malaria Journal, Volume 9, No. 1, Article 129, Year 2010
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Description
Background. Accurate diagnosis is essential for prompt and appropriate treatment of malaria. While rapid diagnostic tests (RDTs) offer great potential to improve malaria diagnosis, the sensitivity of RDTs has been reported to be highly variable. One possible factor contributing to variable test performance is the diversity of parasite antigens. This is of particular concern for Plasmodium falciparum histidine-rich protein 2 (PfHRP2)-detecting RDTs since PfHRP2 has been reported to be highly variable in isolates of the Asia-Pacific region. Methods. The pfhrp2 exon 2 fragment from 458 isolates of P. falciparum collected from 38 countries was amplified and sequenced. For a subset of 80 isolates, the exon 2 fragment of histidine-rich protein 3 (pfhrp3) was also amplified and sequenced. DNA sequence and statistical analysis of the variation observed in these genes was conducted. The potential impact of the pfhrp2 variation on RDT detection rates was examined by analysing the relationship between sequence characteristics of this gene and the results of the WHO product testing of malaria RDTs: Round 1 (2008), for 34 PfHRP2-detecting RDTs. Results. Sequence analysis revealed extensive variations in the number and arrangement of various repeats encoded by the genes in parasite populations world-wide. However, no statistically robust correlation between gene structure and RDT detection rate for P. falciparum parasites at 200 parasites per microlitre was identified. Conclusions. The results suggest that despite extreme sequence variation, diversity of PfHRP2 does not appear to be a major cause of RDT sensitivity variation. © 2010 Baker et al; licensee BioMed Central Ltd.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC2893195/bin/1475-2875-9-129-S1.DOC
https://efashare.b-cdn.net/share/pmc/articles/PMC2893195/bin/1475-2875-9-129-S2.DOC
https://efashare.b-cdn.net/share/pmc/articles/PMC2893195/bin/1475-2875-9-129-S3.DOC
Authors & Co-Authors
Baker, Joanne
Australia, Enoggera
Australian Army Malaria Institute
Australia, Brisbane
The University of Queensland
Ho, Mei Fong
Australia, Enoggera
Australian Army Malaria Institute
Australia, Brisbane
The University of Queensland
Pelecanos, Anita
Australia, Brisbane
The University of Queensland
Australia, Brisbane
Qimr Berghofer Medical Research Institute
Gatton, Michelle L.
Australia, Brisbane
The University of Queensland
Australia, Brisbane
Qimr Berghofer Medical Research Institute
Chen, Nanhua
Australia, Enoggera
Australian Army Malaria Institute
Abdulla, Salim Mohammed K.
Tanzania
Bagamoyo/ifakara Health Research and Development Centre
Albertini, Audrey
Switzerland, Geneve
Foundation for Innovative and new Diagnostics
Ariey, Frédéric
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Barnwell, John W.
United States, Atlanta
Centera for Disease Control
Bell, David R.
Switzerland, Geneve
Foundation for Innovative and new Diagnostics
Switzerland, Geneva
Organisation Mondiale de la Santé
Cunningham, Jane A.
Switzerland, Geneve
Training in Tropical Diseases Tdr
Djallé, Djibrine
Central African Republic, Bangui
Institut Pasteur de Bangui
Echeverry, Diego Fernando
Colombia, Cali
Centro Internacional de Entrenamiento e Investigaciones Medicas
Gamboa Vilela, Dionicia V.
Peru, Lima
Universidad Peruana Cayetano Heredia, Instituto de Medicina Tropical Alexander Von Humboldt
Peru, Lima
Universidad Peruana Cayetano Heredia
Hii, Jeffrey L.K.
Switzerland, Geneva
Organisation Mondiale de la Santé
Kyaw, Myat Phone
Unknown Affiliation
Luchavez, Jennifer
Philippines, Manila
Research Institute for Tropical Medicine
Membi, Christopher D.
Tanzania
Bagamoyo/ifakara Health Research and Development Centre
Ménard, Didier
Madagascar, Antananarivo
Institut Pasteur de Madagascar
Murillo, Claribel
Colombia, Cali
Centro Internacional de Entrenamiento e Investigaciones Medicas
Nhem, Sina
Cambodia, Phnom Penh
Institut Pasteur du Cambodge
Ogutu, Bernhards R.
Kenya, Nairobi
Kenya Medical Research Institute
Onyor, Pamela
Kenya, Nairobi
Kenya Medical Research Institute
Oyibo, Wellington Aghoghovwia
Nigeria, Lagos
College of Medicine, University of Lagos
Wang, Shan Qing
China, Haikou
Hainan Center for Disease Control and Prevention
McCarthy, James S.
Australia, Brisbane
The University of Queensland
Cheng, Qin
Australia, Enoggera
Australian Army Malaria Institute
Australia, Brisbane
Qimr Berghofer Medical Research Institute
Statistics
Citations: 27
Authors: 27
Affiliations: 18
Identifiers
Doi:
10.1186/1475-2875-9-129
e-ISSN:
14752875
Research Areas
Cancer
Genetics And Genomics
Infectious Diseases
Study Approach
Quantitative