Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
agricultural and biological sciences
Multicenter validation of a multiplex PCR assay for differentiating the major Listeria monocytogenes serovars 1/2a, 1/2b, 1/2c, and 4b: Toward an international standard
Journal of Food Protection, Volume 68, No. 12, Year 2005
Notification
URL copied to clipboard!
Description
The performance of a multiplex PCR assay that separates the four major serovars of the pathogenic Listeria monocytogenes into four distinct PCR groups was evaluated through a multicenter typing study. Identical panels of 90 Listeria isolates were distributed to five participating laboratories that were blind to the nature of the isolates. Isolates were characterized using the previously standardized protocol. Overall concordance was 96.6 to 100%, sufficient for the assay to be used as an alternative to serotyping and confidently applied in laboratories involved in L. monocytogenes typing. Copyright ©, International Association for Food Protection.
Authors & Co-Authors
Doumith, Michel
France, Paris
Institut Pasteur, Paris
Jacquet, Christine
France, Paris
Institut Pasteur, Paris
Gerner-Smidt, Peter
Denmark, Copenhagen
Statens Serum Institut
Graves, Lewis M.
United States, Atlanta
Centers for Disease Control and Prevention
Lončarević, Semir
Norway, Oslo
Veterinærinstituttet
Morvan, Anne
France, Paris
Institut Pasteur, Paris
Torpdahl, Mia
Denmark, Copenhagen
Statens Serum Institut
Vázquez, Julio Alberto
United States, Bethesda
National Institutes of Health Nih
Martin, Paul M.V.
France, Paris
Institut Pasteur, Paris
Statistics
Citations: 75
Authors: 9
Affiliations: 5
Identifiers
Doi:
10.4315/0362-028X-68.12.2648
ISSN:
0362028X
Research Areas
Disability
Food Security