Skip to content
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Menu
Home
About Us
Resources
Profiles Metrics
Authors Directory
Institutions Directory
Top Authors
Top Institutions
Top Sponsors
AI Digest
Contact Us
Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
Performance of Multiplex Commercial Kits to Quantify Cytokine and Chemokine Responses in Culture Supernatants from Plasmodium falciparum Stimulations
PLoS ONE, Volume 8, No. 1, Article e52587, Year 2013
Notification
URL copied to clipboard!
Description
Background: Cytokines and chemokines are relevant biomarkers of pathology and immunity to infectious diseases such as malaria. Several commercially available kits based on quantitative suspension array technologies allow the profiling of multiple cytokines and chemokines in small volumes of sample. However, kits are being continuously improved and information on their performance is lacking. Methodology/Principal Findings: Different cytokine/chemokine kits, two flow cytometry-based (eBioscience® FlowCytomix™ and BD™ Cytometric Bead Array Human Enhanced Sensitivity) and four Luminex®-based (Invitrogen™ Human Cytokine 25-Plex Panel, Invitrogen™ Human Cytokine Magnetic 30-Plex Panel, Bio-Rad® Bio-Plex Pro™ Human Cytokine Plex Assay and Millipore™ MILLIPLEX® MAP Plex Kit) were compared. Samples tested were supernatants of peripheral blood mononuclear cells of malaria-exposed children stimulated with Plasmodium falciparum parasite lysates. Number of responses in range that could be detected was determined and reproducibility of duplicates was evaluated by the Bland-Altman test. Luminex® kits performed better than flow cytometry kits in number of responses in range and reproducibility. Luminex® kits were more reproducible when magnetic beads were used. However, within each methodology overall performance depended on the analyte tested in each kit. Within the Luminex® kits, the Invitrogen™ with polystyrene beads had the poorer performance, whereas Invitrogen™ with magnetic beads had the higher percentage of cytokines/chemokines with both readings in range (40%), followed by Bio-Rad® with magnetic beads (35%). Regarding reproducibility, the Millipore™ kit had the highest percentage (60%) of cytokines/chemokines with acceptable limits of agreement (<30%), followed by the Invitrogen™ with magnetic beads (40%) that had tighter limits of agreement. Conclusions/Significance: Currently available kits for cytokine and chemokine quantification differ in reproducibility and concentration range of accurate detection. Luminex®-based kits with magnetic beads perform the best. Data highlights the importance of testing different kits before each study to choose the most appropriate, depending on the priority of the cytokines assessed. © 2013 Moncunill et al.
Available Materials
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s001.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s002.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s003.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s004.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s005.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s006.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s007.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s008.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s009.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s010.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s011.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s012.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s013.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s014.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s015.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s016.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s017.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s018.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s019.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s020.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s021.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s022.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s023.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s024.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s025.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s026.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s027.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s028.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s029.pdf
https://efashare.b-cdn.net/share/pmc/articles/PMC3534665/bin/pone.0052587.s030.pdf
Authors & Co-Authors
Moncunill, Gemma
Spain, Barcelona
Universitat de Barcelona
Aponte, John Jairo
Spain, Barcelona
Universitat de Barcelona
Nhabomba, Augusto J.
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Dobaño, Carlota
Spain, Barcelona
Universitat de Barcelona
Mozambique, Manhica
Centro de Investigação em Saúde de Manhiça Cism
Statistics
Citations: 62
Authors: 4
Affiliations: 2
Identifiers
Doi:
10.1371/journal.pone.0052587
e-ISSN:
19326203
Research Areas
Infectious Diseases
Maternal And Child Health
Study Approach
Quantitative