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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
pharmacology, toxicology and pharmaceutics
New insights into mycotoxin mixtures: The toxicity of low doses of Type B trichothecenes on intestinal epithelial cells is synergistic
Toxicology and Applied Pharmacology, Volume 272, No. 1, Year 2013
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Description
Deoxynivalenol (DON) is the most prevalent trichothecene mycotoxin in crops in Europe and North America. DON is often present with other type B trichothecenes such as 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), nivalenol (NIV) and fusarenon-X (FX). Although the cytotoxicity of individual mycotoxins has been widely studied, data on the toxicity of mycotoxin mixtures are limited. The aim of this study was to assess interactions caused by co-exposure to Type B trichothecenes on intestinal epithelial cells. Proliferating Caco-2 cells were exposed to increasing doses of Type B trichothecenes, alone or in binary or ternary mixtures. The MTT test and neutral red uptake, respectively linked to mitochondrial and lysosomal functions, were used to measure intestinal epithelial cytotoxicity. The five tested mycotoxins had a dose-dependent effect on proliferating enterocytes and could be classified in increasing order of toxicity: 3-ADON. <. 15-ADON. ≈. DON. <. NIV. ≪. FX. Binary or ternary mixtures also showed a dose-dependent effect. At low concentrations (cytotoxic effect between 10 and 30-40%), mycotoxin combinations were synergistic; however DON-NIV-FX mixture showed antagonism. At higher concentrations (cytotoxic effect around 50%), the combinations had an additive or nearly additive effect. These results indicate that the simultaneous presence of low doses of mycotoxins in food commodities and diet may be more toxic than predicted from the mycotoxins alone. Considering the frequent co-occurrence of trichothecenes in the diet and the concentrations of toxins to which consumers are exposed, this synergy should be taken into account. © 2013 Elsevier Inc.
Authors & Co-Authors
Alassane-Kpembi, Imourana
France, Toulouse
Toxicologie Alimentaire - Toxalim
France, Toulouse
École Nationale Vétérinaire de Toulouse
Benin
Institut Des Sciences Biomédicales Appliquées
Kolf-Clauw, Martine
France, Toulouse
Toxicologie Alimentaire - Toxalim
France, Toulouse
École Nationale Vétérinaire de Toulouse
Gauthier, Thierry
France, Toulouse
Toxicologie Alimentaire - Toxalim
France, Toulouse
École Nationale Vétérinaire de Toulouse
Abrami, Roberta
France, Toulouse
Toxicologie Alimentaire - Toxalim
France, Toulouse
École Nationale Vétérinaire de Toulouse
Abiola, François Adebayo
Benin
Institut Des Sciences Biomédicales Appliquées
OSWALD, I. P.
France, Toulouse
Toxicologie Alimentaire - Toxalim
France, Toulouse
École Nationale Vétérinaire de Toulouse
Puel, Olivier
France, Toulouse
Toxicologie Alimentaire - Toxalim
France, Toulouse
École Nationale Vétérinaire de Toulouse
Statistics
Citations: 179
Authors: 7
Affiliations: 3
Identifiers
Doi:
10.1016/j.taap.2013.05.023
ISSN:
0041008X
e-ISSN:
10960333
Research Areas
Environmental
Food Security