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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
immunology and microbiology
Evaluation of the NucliSens EasyQ assay in HIV-1-infected individuals in South Africa
Journal of Virological Methods, Volume 124, No. 1-2, Year 2005
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Description
We compared the performance of the NucliSens EasyQ assay (bioMerieux) combined with the manual NucliSens miniMag extraction methodology to the Roche Cobas Ampliprep/Standard Amplicor Monitor methodology (Roche Diagnostics) for HIV-1 RNA quantitation in HIV-1-infected individuals in South Africa. Plasma samples (284) from HIV sero-positive patients at different stages of infection were analyzed. The distribution of results was typical of the clinical samples received at the laboratory where 20% have viral load results <400 copies/ml (2.6 log) and 18% have viral load results >750 000 copies/ml (5.8 log) using the Roche Amplicor Monitor standard assay. All statistical analyses were performed using log10-transformed values for all the variables in the analyses, i.e. log10EasyQIU/ml, and log10RNA (log 10 copies/ml, Amplicor). Roche values were converted from RNA copies per ml to IU/ml by multiplying the Roche value by 0.51. HIV RNA levels quantitated by the NucliSens EasyQ assay correlated significantly with those of the Roche Cobas Amplicor Monitor assay (r = 0.874, p < 0.0001). Reproducibility of the NucliSens EasyQ assay in the log 6 IU range yielded CV variance of 1.3-2.84% for two well-trained technologists. In addition, a retrospective evaluation of the performance of the NucliSens EasyQ assay in 102 runs (2448) samples was conducted in the laboratory over a 4-month interval. Factors considered during this evaluation included time taken to perform the assay, volume requirements, number of required repeats, potential for contamination. © 2004 Elsevier B.V. All rights reserved.
Authors & Co-Authors
Stevens, W.
South Africa, Parktown
Univ. W.
Wiggill, Tracey M.
South Africa, Parktown
Univ. W.
Horsfield, Pamela
South Africa, Parktown
Univ. W.
Coetzee, Lindi Marie
South Africa, Parktown
Univ. W.
Scott, Lesley Erica
South Africa, Parktown
Univ. W.
Statistics
Citations: 50
Authors: 5
Affiliations: 1
Identifiers
Doi:
10.1016/j.jviromet.2004.11.009
ISSN:
01660934
Research Areas
Infectious Diseases
Study Design
Cohort Study
Study Locations
South Africa