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Publication Details
AFRICAN RESEARCH NEXUS
SHINING A SPOTLIGHT ON AFRICAN RESEARCH
biochemistry, genetics and molecular biology
Global assessment of GU-rich regulatory content and function in the human transcriptome
RNA Biology, Volume 8, No. 4, Year 2011
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Description
Unlike AU-rich elements (AREs) that are largely present in the 3′UTRs of many unstable mammalian mRNAs, the function and abundance of GU-rich elements (GREs) are poorly understood. We performed a genome-wide analysis and found that at least 5% of human genes contain GREs in their 3′UTRs with functional over-representation in genes involved in transcription, nucleic acid metabolism, developmental processes and neurogenesis. GREs have similar sequence clustering patterns with AREs such as overlapping GUUUG pentamers and enrichment in 3′UTRs. Functional analysis using T-cell mRNA expression microarray data confirms correlation with mRNA destabilization. Reporter assays show that compared with AREs the ability of GREs to destabilize mRNA is modest and does not increase with the increasing number of overlapping pentamers. Naturally occurring GREs within U-rich contexts were more potent in destabilizing GFP reporter mRNAs than synthetic GREs with perfectly overlapping pentamers. Overall, we find that GREs bear a resemblance to AREs in sequence patterns but they regulate a different repertoire of genes and have different dynamics of mRNA decay. A dedicated resource on all GRE-containing genes of the human, mouse and rat genomes can be found at brp.kfshrc.edu.sa/GredOrg. © 2011 Landes Bioscience.
Authors & Co-Authors
Halees, Anason
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Hitti, Edward
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
al-Saif, Maher Mohammed
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Mahmoud, Linah Mahmood
Saudi Arabia, Riyadh
King Faisal Specialist Hospital and Research Centre
Vlasova-St Louis, Irina A.
United States, Minneapolis
University of Minnesota Twin Cities
Beisang, Daniel J.
United States, Minneapolis
University of Minnesota Twin Cities
Bohjanen, Paul R.
United States, Minneapolis
University of Minnesota Twin Cities
Khabar, Khalid S.A.
United States, Minneapolis
University of Minnesota Twin Cities
Statistics
Citations: 8
Authors: 8
Affiliations: 2
Identifiers
Doi:
10.4161/rna.8.4.16283
ISSN:
15476286
e-ISSN:
15558584